上海金畔生物提供MO-BIO PowerSoil® DNA Isolation Kit Components

上海金畔生物科技有限公司提供MO-BIO PowerSoil® DNA Isolation Kit Components

数量: 大量
品牌: MO-BIO
供应商: 上海金畔生物
规格: 50T

PowerSoil® DNA Isolation Kit

For isolating genomic microbial DNA of the highest quality and purity from most environmental samples

Features

– Next generation method improves PCR inhibitor removal efficiency

– Processes all soil types, including difficult environmental samples

– Patented technology removes 100% of humic substances and PCR inhibitors

– Rapid protocol enables purification of DNA from 250 mg soil in 30 minutes

– Applications include biodefense, soil-borne pathogen detection, agriculture and zoology research

Description

The PowerSoil® DNA Isolation Kit* is our next generation soil DNA kit, which uses our novel, patented Inhibitor Removal Technology® to extract microbial DNA from all soil types and other environmental samples. The isolated DNA has the highest level of purity allowing for more successful PCR and QPCR amplification of organisms from the sample. The kit is ideal for processing all environmental samples including difficult types containing a high humic acid content such as compost, sediment, and manure. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces), and nematodes.

The PowerSoil® DNA Isolation Kit distinguishes itself from other kits with a patented humic substance/brown color removal procedure that removes PCR inhibitors from even the most difficult soil types, promoting successful downstream PCR analysis. When choosing between the PowerSoil® DNA Isolation Kit and the UltraClean® Soil DNA Isolation Kit, use this kit for samples that are very high in humic content.

Do not let the name fool you. Our customers use this kit successfully on diverse sample types such as food (cheese, milk, meat, fruit), water, insects and fecal samples in a variety of applications including biodefense, agriculture and zoology. These soil kits are used by the USEPA, USDA, FBI and CDC for soil analysis.

Sample Kit

Research and Development

The PowerSoil® DNA Isolation Kit is More Effective for Community Analysis by PCR 

 

High Yield, Intact Genomic DNA
MO BIO
PowerSoil®
Supplier A

Supplier B

 
    M   1   2   3   4   5   6   7   8   9

Total Genomic DNA was isolated from nine different
sample types using the MO BIO PowerSoil® DNA Isolation Kit
and soil DNA isolation kits from two other suppliers. All
isolations were performed following the manufacturerís
protocols. Eluted genomic DNA was displayed on a 0.8%
TAE agarose gel (15 µl per lane).

 

Confidence in Your PCR Analysis
MO BIO
PowerSoil®
Supplier A

Supplier B

   
    M   1   2   3   4   5   6   7   8   9

PCR analysis using eubacterial primers was performed
on 1 µl of the undiluted DNA eluate. PCR products
were displayed on a 0.8% TAE agarose gel.
Lane M is DNA Marker.

Lane 1 - Landfill 0-3 inches Lane 4 - Coffee Compost Lane 7 - Mud Sediment
Lane 2 - Landfill 3-6 inches Lane 5 - Marine Sediment Lane 8 - Horse Manure
Lane 3 - Late-stage Compost Lane 6 - Lake Sediment Lane 9 - Mulch Topsoil

Only DNA isolated with PowerSoil® yielded a PCR product for Landfill samples (lanes 1 and 2), Lake Sediment (lane 6) and Mulch Topsoil (lane 9). DNA isolated using other supplier kits failed to amplify products for these samples, most likely due to high levels of humic acid substances remaining in the final DNA eluate.

Positive PCR amplification using PowerSoil® was observed with 100% of the samples. PCR analysis resulted in only 44% positive amplification with supplier kits tested.

Format Silica Spin Filter Tubes
Method Bead Beating
Binding Capacity Up to 20 µg per filter
Sample Size 250 mg
Throughput 1 – 24 samples
Time 30 minutes
Equipment Required Vortex and Vortex Adapter

Kit Components

Component 12888-50 12888-100
PowerBead Tubes
Solution C1
Solution C2
Solution C3
Solution C4
Solution C5
Solution C6
Spin Filters In Tubes
Collection Tubes
50
1 x 3.3 ml
1 x 14 ml
1 x 11 ml
1 x 72 ml
1 x 27.5 ml
1 x 6 ml
50
200
100
1 x 6.6 ml
1 x 28 ml
1 x 22 ml
1 x 144 ml
1 x 55 ml
1 x 12 ml
100
400