Hampton Polyethylene glycol 4,000

Hampton Polyethylene glycol 4,000

Crystallization grade Polyethylene glycol for formulating screens or for optimizatio
Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Polyethylene glycol 4,000
Synonym: PEG 4,000
Mr 3,500-4,500
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3500000
MDL Number MFCD00081839
PubChem Substance ID 24890217
Melting point (Starting Material) 58.0-61.0°C
Aldehyde None detected
Peroxide None detected

HR2-529 Measured pH range: 6.9 – 9.5 at 25°C
HR2-529 Measured Conductivity Range: 35.3 – 46.9 µS/cm at 25°C
HR2-529 Measured Refractive Index Range: 1.40172 – 1.40279 at 20°C

HR2-605 Polyethylene glycol 4,000 Flake – 500 g
HR2-529 Polyethylene glycol 4,000 50% w/v solution – 200 ml

Hampton结晶用缓冲盐试剂盒– SaltRx

Hampton结晶用缓冲盐试剂盒– SaltRx

SaltRx 1 • SaltRx 2 • SaltRx HT


Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules


Salt versus pH matrix crystallization screen
Samples pH 4 to 9
22 unique salts versus salt concentration and pH
Compatible with microbatch, vapor diffusion, liquid & gel diffusion methods
Tube or Deep Well block format
Preformulated, ready to screen
Developed at Hampton Research
All salts and buffers in screen readily available as Optimize™ reagents for reproducing and optimizing crystals
Individual reagents available through the Hampton Research Custom Shop
SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules. Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH. The selection, concentration, and pH of the salts were determined by data mining the BMCD1 and other crystallization databases, crystallization reports in the literature, and internal crystallization trials performed at Hampton Research. Based on this analysis, up to 35% of protein crystallizations involve salt as the primary crystallization reagent. SaltRx can be used as a primary crystallization screen when salt, ionic strength and pH are desired or suspected as appropriate crystallization variables. It is also useful as a secondary screen when salt only reagents/conditions from screens such as Index™, Crystal Screen™, and Grid Screen™ produce crystals and when further screening for additional salt conditions or optimization is desired. Since this screen does not contain volatile organics, it is compatible with microbatch, vapor diffusion, liquid and gel diffusion crystallization methods. SaltRx may also be used for dialysis crystallization in conjunction with Dialysis Buttons™.The original SaltRx kit (HR2-108) contained 96 unique reagents, 10 ml each. This kit has been discontinued and converted into two 48 reagent based kits, SaltRx 1 and SaltRx 2, available separately, which when used together are identical to the original SaltRx.SaltRx 1 contains 48 unique reagents, 10 ml each. SaltRx 1 contains reagents 1-48 of the original SaltRx 96 reagent kit.Salt Rx 2 contains 48 unique reagents, 10 ml each. SaltRx 2 contains reagent 49-96 of the original SaltRx 96 reagent kit.SaltRx HT contains the 96 SaltRx 1 and SaltRx 2 reagents in a single Deep Well block format.Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Measured pH range of kit is 4.1 to 9.0 at 25°C
Average measured pH of kit is 6.9 at 25°C
Median measured pH of kit is 7.3 at 25°C
Mode measured pH of kit is 7.4 at 25°C

HR2-136 SaltRx HT 1 ml, Deep Well block format
HR2-107 SaltRx 1 10 ml, tube format
HR2-109 SaltRx 2 10 ml, tube format



1.Screens 晶体删选试剂盒

产品名称 货号 用途备注
Index Kit HR2-144 用于生物大分子结晶
CrystalScreen Kit HR2-110 用于蛋白质,核酸水溶性小分子结晶
Crystal Screen 2 Kit HR2-112
Pegrx 1 HR2-082 用于生物大分子结晶
Pegrx 2 HR2-084 用于生物大分子结晶
Peg/Ion Screen Kit HR2-126 用于生物大分子结晶
Peg/Ion 2 Screen™ HR2-098 用于生物大分子结晶
Saltrx Kit HR2-108 用于生物大分子结晶
Membfac Kit HR2-114 用于膜蛋白和溶解度有限的样品结晶
Crystal Screen Lite HR2-128 用于蛋白质,缩氨酸,核酸的结晶
Natrix Kit HR2-116 用于核酸和蛋白核酸复合物的结晶
Crystal Screen Cyro Kit HR2-122 用于蛋白质,可溶性缩氨酸,核酸和水溶性小分子的初次低温结晶
Nucleic Acid Mini Screen Kit HR2-118 用于核酸片段的结晶
Low Ionic Strength Screen HR2-120 用于完整单抗,单抗片段以及在低离子浓度下溶解度较低的样品结晶
Silver Bullet & Silver Bullet Bio HR2-078 用于生物大分子的初次和二次结晶
Peg/Tacsimate Ph5.8 Crystallization Reagent HR2-090
Peg/Tacsimate Ph 6.8 Crystallization Reagent HR2-092
Peg/Tacsimate Ph 7.8 Crystallization Reagent HR2-094
Additive Screen™ HR2-428 利用样品间和样品溶剂间相互作用来优化晶体生长和改变样品溶解度
产品名称 货号 用途备注
Detergent Screen 1 HR2-410 改善疏水作用引起的非特异性聚集
Detergent Screen 2 HR2-411
Detergent Screen 3 HR2-412
Detergent Screen HR2-408
Heavy Atom Screen Pt HR2-442 Heavy Atoms For Multiple Isomorphous Replacement
Heavy Atom Screen Au HR2-444
Heavy Atom Screen Hg HR2-446
Heavy Atom Screen M1 HR2-448
Heavy Atom Screen M2 HR2-450
I3c Phasing Kit HR3-133

2.Custom Shop Crystallization Reagents 结晶试剂盒中单独的试剂

  1. Optimize™ Reagents晶体生长条件优化试剂

⑴ Optimize™ – Polymers聚合体

⑵ Optimize™- Organics (Volatile) 挥发性有机物

⑶ Optimize™- Organics (Non-Volatile)非挥发性的有机物

⑷ Optimize™- Salts 盐类

⑸ Optimize™- Buffers缓冲液

⑹ Optimize™- Solubilizing Agents (NDSB) 溶解试剂NDSB

⑺ Optimize™ – Reducing Agent还原剂-TCEP氢氯化物

⑻ Optimize™ – Cryoprotectants

⑼ Optimize™ – Oils用于结晶的油类

⑽ Optimize™ – Silica Hydrogel硅胶

⑾ Optimize™ – LM Agarose低熔点琼脂糖
5.Crystallization Plates, Hardware & Accessories结晶耗材
⑴ 24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种
⑵ 48 Well Crystallization Plates 48孔晶体板
⑶ 72 Well Crystallization Plates72孔晶体板
⑷ 96 Well Crystallization Plates 96孔晶体板等

Hampton 24孔悬滴板 VDX Plate with sealant(带密封剂)

Hampton 24孔悬滴板 VDX Plate with sealant(带密封剂)
VDX Plate with sealant

Hanging drop crystallization
Sitting drop crystallization with Micro-Bridges® or Glass Sitting Drop Rods™
Dialysis crystallization with Dialysis Buttons™

24 well plate with raised cover
Sealant applied to 24 wells, ready to seal with cover slides
Compatible with 22 mm diameter square and circle cover slides
Optically clear plastic
Extremely versatile and cost-effective crystallization platform
Compatible with UV crystal imaging when used with HR3-231 and HR3-233 – 22 mm x 0.22 mm Siliconized circle cover slides and HR3-215 and HR3-217 – 22 mm x 0.22 mm Siliconized square cover slides

24 well crystallization plate for hanging drop or sitting drop vapor diffusion crystallization (when used with Micro-Bridges or Glass Rods), or dialysis crystallization (when used with Dialysis Buttons). Stackable, optically clear plastic 24 well plates with raised covers (to allow room for cover slides) and flat bottoms for exceptional optics. Raised, wide rings around each reservoir (well) minimize cross contamination and allow each well to be individually sealed with 22 mm diameter circle or square cover slides. Plates are individually wrapped and supplied with applied sealant. 15.0 cm x 10.8 cm plate footprint is convenient for manual pipetting and well access while at the same time compatible with some automated liquid handling systems. Approximate dimensions: 15.0 cm x 10.6 cm x 2.2 cm. Approximate well size: 1.7 cm x 1.6 cm. Typical well volume: 500 to 1,000 µl. Well capacity: 3.5 ml.

HR3-172 VDX邃「 Plate with sealant 24 plate case
HR3-170 VDX邃「 Plate with sealant 100 plate case

Hampton 蛋白质结晶试剂和耗材介绍


Hampton 蛋白质结晶试剂和耗材介绍

美国Hampton Research公司位于加利福尼亚州,是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材,同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线,不仅为全世界晶体研究人员的工作带来了便捷,而且也受益于科研人员的回馈不断发展自己,目前已成为晶体研究领域最受信赖的品牌之一。
上海金畔生物作为Hampton research品牌蛋白结晶产品的代理商,我们将竭诚为您服务,欢迎新老客户咨询。

Hampton research主要产品:

产品分类 特点描述
Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液
Optimize Reagents 结晶级别盐类,聚合物,有机溶液或缓冲液
Crystallization Plates, Hardware & Accessories 结晶板(悬滴法和坐滴法,微量透析,微配液,自由界面扩散,凝胶以及无容器悬浮法)及其他配件
Cryocrystallography 低温晶体学耗材如Crystalcap system,cryoloops
Goniometer Heads&Supplies 测角器头和配件
Protein Crystallization Standards 结晶级别标准蛋白
Custom Shop

Crystallization Reagents

StockOptions Kits 内含盐类,缓冲液和冷冻液的简便型试剂盒
Tools,Seeding&Resin 结晶用探针,结晶笔,seeding法结晶工具
Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂

美国Hampton Research公司是世界上著名的蛋白质晶体试剂供应商,该公司的产品目录是由上海金畔生物科技有限公司提供,该目录分为12个大类和几十个小类。


第一类:Crystallization Screens   用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液

1.PCT Pre-Crystallization Test(PCT预结晶试验)


  1. Slice pH(PH切片)


  1. Solubility & Stability Screen(溶解度和稳定性的屏幕)


  1. Index


  1. Crystal Screen(晶体屏幕)



5.PEG/Ion Screen & PEG/Ion HT Screen 用于可溶性蛋白的结晶

6.PEG/Ion 2 Screen?用于可溶性生物大分子结晶

7.Crystal Screen? & Crystal Screen? HT 用于蛋白质,复合物,缩氨酸,核酸以及水溶性小分子的传统的经典结晶试剂

8.Crystal Screen 2?用于蛋白质,缩氨酸,核酸以及水溶性小分子的结晶

9.Crystal Screen Lite?用于蛋白质,缩氨酸和核酸的结晶试剂

10.Crystal Screen Cryo?用于蛋白质,缩氨酸,核酸的结晶

11.Grid ScreenTM用于蛋白质的结晶,分为七小类。

12.Quik Screen?用于可溶性蛋白的结晶

13.Quik Optimize?快速优化试剂,与Quik Screen? 或 custom screen配套使用

14.Low Ionic Strength Screen?用于完整的单克隆抗体,单克隆抗体片段以及低离子浓度很少溶解的蛋白的结晶

15.Nucleic Acid Mini Screen?用于核酸片段的结晶化


17.MembFac?& MembFac? HT用于膜蛋白的结晶

18.Additive Screen?增强或改变样品可溶性的试剂

19.Detergent Screen?去垢剂

20.Heavy Atom Screens重原子结晶试剂

21.Izit Crystal Dye?蛋白染色试剂,用于区分蛋白晶体和盐晶体

第二类:Custom Shop Crystallization Reagents  结晶试剂盒中单独的试剂

  1. Solubility & Stability Individual Reagents
  2. Slice pH Individual Reagents
  3. Ionic Liquid Screen Individual Reagents
  4. Proti-Ace & Proti-Ace 2 Individual Reagents
  5. PCT Individual Reagents
  6. Silver Bullets & Silver Bullets HT Individual Reagents
  7. Silver Bullets Bio & Silver Bullets Bio HT Individual Reagents
  8. Index • Index HT Individual Reagents
  9. PEGRx 1 Individual Reagents
  10. PEGRx 2 Individual Reagents
  11. SaltRx Individual Reagents
  12. PEG/Ion Individual Reagents
  13. Crystal Screen Lite Individual Reagents
  14. Crystal Screen Cryo Individual Reagents
  15. Crystal Screen 2 Cryo Individual Reagents
  16. Grid Screen Ammonium Sulfate Individual Reagents
  17. Grid Screen PEG 6000 Individual Reagent
  18. Grid Screen Sodium Chloride Individual Reagents
  19. Grid Screen PEG/LiCl Individual Reagents
  20. Quik Screen Individual Reagents
  21. Natrix Individual Reagents

第三类:Optimize Reagents 优化试剂


2.Optimize-Organics (Volatile) 挥发性有机物

3.Optimize-Organics (Non-Volatile)非挥发性的有机物

4.Optimize-Salts 盐类


6.Optimize-Solubilizing Agents (NDSB) 溶解试剂NDSB

7.Optimize-Reducing Agent还原剂-TCEP氢氯化物



10.Optimize-Silica Hydrogel硅胶

11.Optimize-LM Agarose低熔点琼脂糖

第四类:StockOptions Kits

  1. StockOptions Salt
  2. StockOptions pH
  3. StockOptions Buffer
  4. CryoPro

第五类:Crystallization Plates, Hardware & Accessories 结晶耗材

1.24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种

2.48 Well Crystallization Plates 48孔晶体板

3.72 Well Crystallization Plates72孔晶体板

4.96 Well Crystallization Plates 96孔晶体板

5.384 Well Crystallization Plates 384孔晶体板-用于坐滴的结晶

6.Cover Slides & Related Tools

7.Glass Sitting Drop Rods用于坐滴的结晶


9.Glass Plates – 3 and 9 Well3孔和9孔玻璃板

10.Sealants, Sealing Grease, Oils, Applicator密封油脂,油及其它器具

11.Sealing Films, Tapes, Mats & Covers

12.Microdialysis Buttons, Rods & Membranes微量渗析结晶化

13.Granada Crystallization Box

第六类:Tools, Seeding & Resin(结晶工具、树脂)

  1. Tungsten-carbide Glass Cutter
  2. X-Acto Gripster Knife
  3. Micro-Tools™ Set
  4. Micro-Tools II™ Set
  5. Forceps
  6. Crystal Probe™
  7. Crystal Pencil
  8. Seed Bead
  9. Seeding Tool


  1. CrystalCap HT™ Systems
  2. CrystalCap Magnetic™ Systems
  3. CrystalCap™ Systems
  4. CryoLoops & MicroTubes
  5. Cryo Tools
  6. Canes, Sleeves, Coders & Boxes
  7. Dewars

第八类:Capillary Mounts & Supplies(毛细管座椅支架供应)

第九类:Goniometer Heads & Supplies(测角头及用品)

第十类:Xenon Derivatization(氙气衍生物)

第十一类:Labels & Pens(标签和记录笔)

第十二类:Protein Crystallization Standards(蛋白质结晶的标准)


Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理(活性荧光染料)   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理(蛋白结晶) whatman代理(滤膜滤纸) GE代理(蛋白纯化) Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理(糖蛋白分析产品)  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

Hampton Solubility & Stability Screen

Hampton Solubility & Stability Screen

Solubility & Stability Screen(溶解度和稳定性的屏幕)


Solubility & Stability Screen

Solubility & Stability Screen

Solubility & Stability Screen

Solubility & Stability Screen HR2-072


A solubility screen, a stability screen, an additive screen for protein assays including ThermoFluor and crystallization
Preformulation screening


Compatible with Thermofluor , Differential Scanning Fluorimetry (DSF), and Protein Thermal Shift assays
96 sterile filtered reagents
17 classes of reagents and excipients
Highly concentrated (10x) reagent formulation
Developed at Hampton Research



Thermal shift assay
Dynamic Light Scattering
Use Solubility & Stability Screen along side Slice pH and Solubility & Stability Screen 2 to optimize sample buffer

Solubility & Stability Screen is designed to assist in the identification of solution conditions which promote protein solubility and stability, and minimize protein precipitation. Solubility & Stability Screen is a solubility screen, a stability screen, and may also be used as an additive screen in the presence of a crystallization reagent.

The Hampton Research Solubility and Stability Screen can evaluate protein solubility, stability and crystallization in the presence of 94 different chemical additives sampling 17 different classes of reagents plus two controls.

Protein solubility and stability are universally required in a wide range of applications, including general biochemical studies, the preparation of proteins in pharmaceuticals, structural biology and crystallization.1-11The preparation of a concentrated, soluble and stable protein sample can often be a difficult task as proteins often aggregate, precipitate or denature.

Thermofluor (also known as Differential Scanning Fluorimetry, DSF and Protein Thermal Shift) provides a rapid, convenient and effective means of identifying subsaturating solution conditions that may also correlate with protein heterogeneity and poor crystallization outcomes.12

Protein solubility and stability is affected by many different chemical factors including pH, buffer type, chemical additives and excipients. pH and buffer type are dominant protein solubility and stability variables and can be evaluated and optimized using the Hampton Research Slice pH kit HR2-070. Slice pH evaluates protein solubility, stability and crystallization versus 20 different buffers over the pH range 3.5-9.6. Chemical additives influencing protein solubility and stability can be evaluated using the Solubility & Stability Screen.

It is widely accepted that protein solubility and stability can be increased by the use of chemical additives.5,15Seventeen classes of reagents are sampled by the Solubility & Stability Screen and each of these classes has been reported as important in improving sample solubility and stability.2-11

The Solubility & Stability Screen is a set of 94 high purity reagents formulated in high purity water (NCCLS/ASTM Type 1+) at 25°C and are 0.22 micron sterile filtered. The 94 solubility and stability reagents are formulated at 2 to 10 times their recommended working concentration. The remaining two reagents are water and a negative (TCA) control. A water control demonstrates the effect of diluting sample as well as sample buffer concentration. TCA, the negative control, demonstrates total sample precipitation, loss of sample solubility and loss of sample stability. The effects of the Solubility & Stability reagents can be compared with this negative control to assist in the identification of reagents promoting sample solubility and stability. 500 microliters of each reagent is supplied in a sterile 96 well polypropylene Deep Well block. The Solubility & Stability Screen reagents are compatible with the sitting or hanging drop vapor diffusion, microbatch, free interface diffusion, sandwich drop vapor diffusion, and dialysis crystallization methods utilizing water soluble reagents.




Cryocrystallography  低温结晶学


SSRL style sample mount for cryocrystallography
CrystalCap attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
Conical shape with ledge compatible with SSRL style grippers, auto mounters and sample handlers

The CrystalCap is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept SSRL style sample mounts. The CrystalCap attaches to a magnetic CrystalCap Vial and Magnetic Goniometer Base. The tip of the CrystalCap accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.

The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage.The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.

The conical shape with ledge is compatible with SSRL style grippers, auto mounters and sample handlers.

Compatible with the following Synchrotron Radiation Beamlines

North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• National Synchrotron Light Source, Upton, New York NSLS X4C
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1

Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A
• Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU




CrystalCap & Vial



Hampton蛋白结晶小瓶和结晶柱底座 CrystalCap™

HR4-733 CrystalCap with Vial – 60 pack
HR4-902 CrystalCap without Vial – 60 pack
HR4-904 CrystalCap Vial Vial only – 30 pack

Hampton 蛋白结晶向导管Wizard Tube

Wizard Tube 
The Wizard™ Classic line of random sparse matrix screens is designed to increase your probability of producing crystals during the coarse screening phase when crystallizing biological macromolecules (proteins, nucleic acids, peptides, and combinations thereof).
The Wizard Classic reagents are proven to be a highly effective starting point in the screening of biological macromolecules. The Wizard Classic formulations include a large range of crystallants, buffers, and salts covering a broad range of crystallization space at pH levels from pH 4.5 to pH 10.5.Choose from Wizard Classic 1, 2, 3, or 4 non-overlapping formulations in matrix blocks or tubes.


Wizard Cubic LCP
The tools in the Wizard Cubic LCP (lipidic cubic phase) Kit enable researchers to prepare LCP-type crystallizations by hand. Ideal for low-protein experiments: effective protein volume for a single crystallization experiment is about 80 nanoliters. Wizard Cubic LCP Kit tools work especially well when traditional methods have failed to yield crystals. Lipidic cubic phase has worked well for the crystallization of 7TM membrane proteins (proteins with seven transmembrane helices). Four out of six GPCRs (G-protein coupled receptors), an important membrane protein class, and several microbial 7TM proteins have been crystallized using the LCP approach.


The Wizard Cryo™ line of random sparse matrix screens is designed for scientists who want to avoid the additional step of optimizing a cryoprotectant condition. Every Wizard Cryo formulation flash-freezes to a clear, amorphous glass in liquid nitrogen or in a cryo-stream at 100K. Crystals can be frozen directly from their growth drops, avoiding the additional step of pre-equilibration with an artificial cryo-condition that can damage the crystal. Eleven different cryocrystallants and sparing use of glycerol ensures a broad sampling of possible cryo conditions. Choose from Wizard Cryo 1 or 2 formulations in tubes or Wizard Cryo 1 and 2 together in a 96-well matrix block.


Hampton 银弹 Silver Bullets

Hampton 银弹 Silver Bullets


Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability


Developed at Hampton Research
Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3

Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3

X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.

Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests

The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:

•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal

The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ºC, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.

Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.

Silver Bullets reagent volume is 0.5 ml (each well).

ThermoFluor is a registered trademark of Johnson & Johnson.

Hampton  Silver Bullets Bio

Silver Bullets • Silver Bullets Bio

Silver Bullets • Silver Bullets Bio


Applications 应用

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability


Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates


Developed at Hampton Research
Two sets of 96 reagents, composed of more than 1,090 chemicals, of which more than 400 are uniqu

Please note, the Silver Bullets Bio Screen is temporarily unavailable as a new formulation is in development