Hampton GRAS 1 • GRAS 2 Screens

Hampton GRAS 1 • GRAS 2 Screens

stallization screen for proteins, including monoclonal antibodies, where Polyethylene glycol is the primary, and Salt the secondary reagent, sampling pH 4-9
GRAS 1 • GRAS 2 ScreensGRAS 1 • GRAS 2 Screens Identify GRAS based reagents that promote crystallization of biotherapeutics for bioprocess, bioformulation, and continuous flow manufacturing applications
Features

Developed at Hampton Research
Generally Recognized As Safe reagent formulation
Samples pH 4.0 – 9.0 without an added buffer
Primary crystallization reagent:

GRAS Screen 1: PEG 300, 400, MME 550, & 600
GRAS Screen 2: PEG 1,000, MME 2,000, 3,350 & 4,000
Secondary crystallization reagent:

24 unique salts
Compatible with Vapor diffusion, microbatch, free interface diffusion
Reagents soluble between 4°C and 30°C

Description
GRAS Screen™ 1 and GRAS Screen™ 2 were developed by Hampton Research for the crystallization of proteins, including monoclonal antibodies. Each of the chemicals in GRAS Screen 1 and 2 have been used under one or more of the following categories. As (1) a Generally Recognized As Safe (GRAS) substance, (2) a pharmaceutical excipient, (3) a normal physiological constituent, (4) a metabolic byproduct, and/or (5) a Everything Added to Food in the United States (EAFUS) substance.

GRAS Screen 1 samples four low molecular weight Polyethylene glycols (300, 400, MME 550, and 600) versus twenty-four salts, encompassing pH 4-9. GRAS Screen 1 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 1 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

GRAS Screen 2 samples four medium molecular weight Polyethylene glycols (1,000, MME 2,000, 3,350, & 4,000) versus twenty-four salts, encompassing pH 4-9. GRAS Screen 2 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 2 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

CAT NO NAME DESCRIPTION
HR2-451 GRAS Screen 1 1 ml, Deep Well block format
Price Quantity
$245.00
CAT NO NAME DESCRIPTION
HR2-452 GRAS Screen 2 1 ml, Deep Well block format
Price Quantity
$245.00

Hampton 水晶透明密封带Crystal Clear Sealing Tape

Hampton 水晶透明密封带Crystal Clear Sealing Tape

Crystal Clear Sealing Tape

3 inch, 1.88 inch, 1.88 inch with dispenser, 0.75 inch with dispenser (left to right)

Crystal Clear Sealing Tape

0.75 inch Crystal Clear Sealing Tape

Applications  应用
Sealing tape used to seal sitting drop crystallization experiments

Description

These optically clear tapes are compatible with protein crystallization reagents. The polypropylene Crystal Clear Sealing Tapes use acrylic solvent based adhesives which are compatible with aqueous crystallization reagents.

Catalog number HR3-511 is a 1.88 inch (48 mm) wide, 3 mil tape on a 43.7 yard (40 M) roll with a 1.5 inch core and is supplied with a green dispenser / cutter.Catalog number HR4-511 is a 1.88 inch wide, 3 mil tape on a 60 yard roll with a 3 inch core and no dispenser / cutter.Two strips of the HR3-511 or HR4-511 will seal a Corning, Cryschem, CrystalClear Strip, Greiner, Intelliplate, Linbro, Swissci (MRC), VDX and VDXm plate.Catalog number HR4-506 is a 3 inch wide, 3 mil tape on a 54.86 yard roll with a 3 inch core and no dispenser / cutter.One strip of the HR4-506 will seal a Corning, Cryschem M, CrystalClear Strip, Greiner, Intelliplate, Swissci (MRC) and VDXm plate.Crystal Clear Mini Sealing Tape HR4-508 is a 0.75 inch (19 mm) wide, 3 mil tape on a 650 inch (16.5 M) roll and is supplied with a clear dispenser / cutter. The tape is useful for resealing or patching wells after mounting crystals. The tape is also wide enough to seal a single row of a Cryschem S or Cryschem M plate.See chart for tape and plate compatibility.

Hampton 48孔坐滴蛋白结晶板 48-Well Crystallization Plate

Hampton 48孔坐滴蛋白结晶板 48-Well Crystallization Plate
Applications

Sitting drop crystallization
Features

One drop per reservoir
SBS format
48 well plate
9 mm standard distance between wells
Drop volume: Up to 10 µl
Reservoir volume: 50 to 200 µl
Micro-numbering alongside drop volumes
Rigid plate structure
Wide partition walls between wells improve sealing
Developed in conjunction with the MRC Laboratory of Molecular Biology in Cambridge, United Kingdom
UV compatible (UVP)
Description
MRC Maxi 48-Well Crystallization Plate for Automated Optimization

The MRC Maxi optimization plate is a breakthrough for macromolecular crystallization presented in a 48 well format. Offering easy to automate crystallization optimization with large sitting-drops, the new MRC Maxi Crystallization plate is the perfect solution. Manufactured by Swissci AG, the plate offers an SBS format while providing 48 wells. MRC Maxi is intended for large drops and is compatible both with standard robotic systems as well as manual pipetting.

The plate was developed at the MRC Laboratory of Molecular Biology (Cambridge, UK) in collaboration with Jan Löwe and Fabrice Gorrec. It is a result of many years of experience in successful robotic high-throughput crystallization and complements the original MRC crystallization plate, which is intended for smaller drop volumes and higher throughput during screening.

Drop volumes of up to 10 µl are possible. The 9 mm standard distance between wells is preserved, enabling the use of multi-channel manual pipettes and robotic liquid handlers, making MRC Maxi one of the most automation-friendly optimization plates on the market.

MRC Maxi is covered by global intellectual property and design registration as are the Swissci AG MRC 2 lens 96 well plates. Several breakthrough features of the original MRC plate have been maintained. Wells are labeled individually. Drops are raised for easy access during crystal retrieval. MRC Maxi uses the same proprietary polymer specially selected for the purpose of UV light visualization and the material used keeps through-plastic evaporation to a minimum. Well shapes are spherical but shallow.

The MRC Maxi Crystallization plate offers unique properties that make it the ideal choice for microliter-sized optimization experiments and is made from UV compatible UVP.

The advantages of the MRC Maxi Crystallization plate – in brief

Easy Crystal Retrieval
Raised wide wells make the crystal mounting especially easy.

Easy Viewing
The wells are wide conical.
Each well has a micro lens for perfect illumination.
Micro numbering readable under the microscope for each well.
The optically superior polymer (UVP) is UV transmissible.

Better Sealing
Wide partition walls between the wells give plenty of area for good sealing with tape.

Very Rigid, Automation-Friendly Plate Design
The UVP polymer reduces through-plastic evaporation to a minimum.

SBS Standard
The plates are designed to the SBS standard and are compatible with all common holders.
9 mm distance from well-to-well within columns, 18 mm distance within rows.

Unique Polymer (UVP)
Ultra-low sample binding.
No static charging.

Recommended Volumes
Volumes validated for MRC Maxi are up to 10 µl of sample drop and 200 µl of the crystallization reagent.

CAT NO NAME DESCRIPTION
HR3-179 MRC Maxi 48-Well Crystallization Plate 10 plate case
HR3-180 MRC Maxi 48-Well Crystallization Plate 40 plate case

Hampton Siliconized Glass Cover Slides

Hampton Siliconized Glass Cover Slides

ApplicationsHanging, sitting or sandwich drop crystallization
FeaturesHigh purity of glassSiliconized, hydrophobic glass surface0.22 mm & 0.96 mm glass thicknessThicker glass slides preserve temperature longer12 mm, 18 mm, & 22 mm glass diameterCircle & squareUV Compatible (>85% transmission)
12 x 0.22 mm Circle
18 x 0.22 mm Circle
22 x 0.22 mm Circle & Square

Description
Siliconized glass cover slides allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution. These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use Dow Corning Vacuum Grease (HR3-510), Dow Corning 7 Release Compound (HR3-508), or immersion oil to seal cover slide to plate. Available in 12, 18 or 22 mm diameter circles and 22 mm diameter squares. Available in 0.22 mm or 0.96 mm glass thickness. The 0.96 mm thick slides are virtually unbreakable during crystallization handling.

The following slides are UV transmissable (>85%):
HR3-277 and HR3-279 – 12 mm x 0.22 mm Siliconized circle cover slides
HR3-231 and HR3-233 – 22 mm x 0.22 mm Siliconized circle cover slides
HR3-239 and HR3-241 – 18 mm x 0.22 mm Siliconized circle cover slides
HR3-215 and HR3-217 – 22 mm x 0.22 mm Siliconized square cover slides

图片

图片
Plain Glass Cover Slides
ApplicationsHanging, sitting or sandwich drop crystallization
Features
0.22 mm glass thickness
12 mm glass diameter
Hydrophilic glass surface
Circle & squareDescription
Plain, non-siliconized, hydrophilic glass cover slides. Useful for hanging drop, sitting drop, and sandwich drop vapor diffusion crystallization methods. The hydrophilic surface creates a drop which is more flat than a drop on a siliconized surface. This offers enhanced imaging for very small drops. Use Dow Corning® Vacuum Grease (HR3-510), Dow Corning® 7 Release Compound (HR3-508), or immersion oil to seal cover slide to plate.

HR3-209T 12 mm Plain circle cover slides for Tecan robot 5 oz case (~2,400 slides)

图片

图片
HR3-209T 12 mm Plain circle cover slides for Tecan robot 5 oz case (~2,400 slides)
PEN-VAC Kit
ApplicationsHolding and manipulating small, flat surfaces such as glass cover slides
FeaturesSelf-contained vacuumNo power neededCompactDescription
PEN-VAC is a great tool for handling small, flat surfaced objects such as plain and siliconized cover slides, as well as plastic slides. Simply depress the side button, touch the tip to the object to be lifted and release the button. PEN-VAC creates a totally self-contained vacuum that lifts up to 50 grams for up to one minute. Less than an ounce, the lightweight aluminum body fits in a pocket. No power supply needed. The kit includes one PEN-VAC with 5 3/4″ blue body, (3) angled and (3) straight probes with (2) each 1/8”, 1/4”, 3/8” diameter blue silicone vacuum cups . It is packaged in a clear plastic storage case.

图片
HR3-251  PEN-VAC Kit

Hampton AlumaSeal™ II Sealing Film and Applicator

Hampton AlumaSeal™ II Sealing Film and Applicator

Applications

Sealing film used to reseal HT format screen kits in polypropylene blocks and plates

Features

Excellent seal
Film conforms to raised chimney wells
Easily pierceable with single or multichannel pipettors and robotic probes
Heat & cold resistant, recommended for temperatures from -80 °C to +120 °C
Certified DNase-, RNase-, and nucleic-acid-free
Less evaporation than clear films
Excellent barrier properties, virtually no reagent evaporation or drying
 Description
A 38 µm soft non-permeable aluminum foil sealing film with strong medical-grade adhesive, AlumaSeal II sealing films eliminate the need for heat-sealing devices or mats during the resealing of reagents in polypropylene deep well blocks. Each sealing film measures 82.6 x 142.9 mm and offers sufficient sealing area for 96 deep well blocks. Length between the perforations with end tabs removed is 125.4 mm. Compared to other aluminum foils, AlumaSeal II has less tendency to roll back on itself when removing the backing paper and it conforms well to the plate during application.AlumaSeal II is a soft, pierceable adhesive film designed for the convenient and rapid sealing of polypropylene deep well blocks. A multiple split backing with two end tabs allows for easy, accurate positioning and secure sealing. The use of an adhesive sealing film minimizes evaporation and helps to prevent well-to-well cross contamination in reagent blocks. AlumaSeal II films are easily pierced by pipettte tips or robotic probes or piercing tools for direct reagent recovery without significant gumming by adhesive.

AlumaSeal™ II Sealing Film and Applicator

AlumaSeal™ II Sealing Film and Applicator

CAT NO NAME DESCRIPTION
HR8-069 AlumaSeal II Sealing Film 100 pack
HR4-413 Film Sealing Paddle 5 pack

Hampton蛋白结晶产品目录

Hampton蛋白结晶产品目录

产品编号 中文品名 英文品名
HR4-126 11 刀片替换装 Replacement #11 Blade
HR4-904 CrystalCap Magnetic Vial CrystalCap™, Vial only
HR4-729 CrystalWand Magnetic, Straight CrystalWand™ Magnetic, Straigh
HR4-837 Micro-Tools II Set Micro-Tools II™ Set
HR4-811 Micro-Tools Set Micro-Tools™ Set
HR3-172 VDX 24孔板,带密封剂 VDX™ Plate, with sealant
HR4-902 带磁力底座 CrystalCap™, without Vial
HR4-733 带磁力底座 CrystalCap™, with Vial
HR4-745 带铜管磁力底座 CrystalCap™ Copper 18 mm, with
HR4-708 冻存套 CryoSleeve™
HR3-217 硅化方形盖玻片22mmx0.22mm 22 mm x 0.22 mm Siliconized Sq
HR4-671 夹钳-弯 Vial Clamp™ – Curved, 45°/135°
HR4-670 夹钳-直 Vial Clamp™ – Straight
HR4-316 Super Glue
HR4-506 胶带 3 inch wide x 55 yard Crystal
HR3-083 结晶板 2孔 MRC 2 Well Crystallization Pla
HR3-125 结晶板 3孔 3 Well Crystallization Plate (
HR4-217 晶体探针 Crystal Probe
HR4-662 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR4-673 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR4-674 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR4-675 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR1-008 液氮罐 CX100 (含运输箱) CX100 Cryo-Express Dry Shipper
HR2-912-37   Crystal Screen 2™ #37
HR2-501   50% v/v Jeffamine® M-600® pH 7
HR2-509   1.0 M BICINE
HR2-517   100% Polyethylene glycol 300
HR2-523   50% w/v Polyethylene glycol 1,
HR2-525   50% w/v Polyethylene glycol 1,
HR2-529   50% w/v Polyethylene glycol 4,
HR2-533   50% w/v Polyethylene glycol 6,
HR2-537   1.0 M Magnesium formate dihydr
HR2-541   3.5 M Ammonium sulfate
HR2-545   2.0 M Lithium sulfate monohydr
HR2-547   7.0 M Sodium formate
HR2-549   1.6 M Sodium citrate tribasic
HR2-551   4.0 M Sodium phosphate monobas
HR2-557   2.0 M Calcium chloride dihydra
HR2-563   1.0 M Zinc acetate dihydrate
HR2-102   StockOptions™ Hepes
HR2-103   StockOptions™ Bis-Tris Propane
HR2-104   StockOptions™ Citric Acid
HR2-106   StockOptions™ Bis-Tris
HR2-107   SaltRx™ 1
HR2-109   SaltRx™ 2
HR2-110   Crystal Screen™
HR2-112   Crystal Screen 2™
HR2-114   MembFac™
HR2-116   Natrix™
HR2-117   Natrix™ 2
HR2-118   Nucleic Acid Mini Screen™
HR2-126   PEG/Ion Screen™
HR2-235   StockOptions™ Sodium Citrate
HR2-237   StockOptions™ Tris Hydrochlori
HR2-239   StockOptions™ Sodium Cacodylat
HR2-241   StockOptions™ pH
HR2-245   StockOptions™ Salt
HR2-249   StockOptions™ Succinic Acid
HR2-320   Seed Bead™
HR2-406   Detergent Screen HT™
HR2-428   Additive Screen™
HR2-434   Reductive Alylation Kit
HR2-442   Heavy Atom Screen™ Pt
HR2-444   Heavy Atom Screen™ Au
HR2-446   Heavy Atom Screen™ Hg
HR2-448   Heavy Atom Screen M1™
HR2-450   Heavy Atom Screen M2™
HR2-082   PEGRx™ 1
HR2-084   PEGRx™ 2
HR2-096   Silver Bullets™
HR2-098   PEG/Ion 2 Screen™
HR4-315   CrystalWand™ Magnetic, 45° McM
HR4-350   Large Applicator (200 & 350 µl
HR4-625   Mounted CryoLoop™ – 20 micron,
HR4-637   CryoTong™ 18 mm (standard 110
HR4-709   CryoCane™ 5 Vial Holder
HR4-711   CryoCane™ 6 Vial Holder
HR2-211   Grid Screen™ Ammonium Sulfate
HR2-213   Grid Screen™ PEG 6000
HR2-227   StockOptions™ Polymer
HR2-233   StockOptions™ Sodium Acetate
HR4-931   CryoLoop™ – 20 micron, 0.2 – 0
HR4-945   Mounted CryoLoop™ – 20 micron,
HR4-947   Mounted CryoLoop™ – 20 micron,
HR4-953   Mounted CryoLoop™ Sampler – 20
HR4-955   Mounted CryoLoop™ – 20 micron,
HR4-957   Mounted CryoLoop™ – 20 micron,
HR4-959   Mounted CryoLoop™ – 20 micron,
HR4-961   Mounted CryoLoop™ – 20 micron,
HR4-963   Mounted CryoLoop™ – 20 micron,
HR4-970   Mounted CryoLoop™ – 20 micron,
HR4-971   Mounted CryoLoop™ – 20 micron,
HR4-989   CryoLoop™ – 10 micron, 0.3 – 0
HR2-128   Crystal Screen Lite™
HR2-138   Additive Screen HT™
HR2-140   PCT™ – Pre-Crystallization T
HR4-313   Mounted CryoLoop™ – 20 micron,
HR2-601   100% Polyethylene glycol 200
HR2-607   50% w/v Polyethylene glycol 10
HR2-609   30% w/v Polyethylene glycol 20
HR2-611   100% Polyethylene glycol monom
HR2-613   50% w/v Polyethylene glycol mo
HR2-615   50% w/v Polyethylene glycol mo
HR2-625   6.0 M 1,6-Hexanediol
HR2-637   5.0 M Sodium chloride
HR2-723   1.0 M BICINE pH 9.0
HR2-755   100% Tacsimate™ pH 7.0
HR2-761   3.0 M DL-Malic acid pH 7.0
HR2-801   TCEP hydrochloride
HR2-821   2.0 M Magnesium sulfate heptah
HR2-829   100% Tacsimate™ pH 8.0
HR2-857   4.0 M TMAO
HR2-859   100% Polyethylene glycol 600
HR2-910-08   Crystal Screen™ #08
HR2-144   Index™
HR4-993   Mounted CryoLoop™ Sampler – 10
HR4-995   Mounted CryoLoop™ – 10 micron,
HR4-999   Mounted CryoLoop™ – 10 micron,
HR6-146   1.0 mm Quartz Capillary
HR8-034   Cryo Puck Carrier
HR8-035   Porcupine Push Tool
HR8-036   Puck Wand
HR8-037   Cryo Puck Base Removal Platfor
HR8-038   Angled Cryo Puck Tong
HR8-039   Cryo Puck Dewar Loading Tool
HR8-114   CrystalCap HT™, Vial only
HR8-133   Seeding Tool
HR8-173   CrystalCap™ Copper HT, 0.025 -
HR8-174   CrystalCap™ Copper HT, 0.05 -
HR8-175   CrystalCap™ Copper HT, 0.1 – 0
HR8-176   CrystalCap™ Copper HT, 0.2 – 0
HR2-575   1.0 M Sodium cacodylate trihyd
HR2-581   1.0 M Hydrochloric acid

Hampton Optimize Reagents

Hampton Optimize Reagents

1、Optimize – Polymers

Crystallization grade polymers for formulating screens or optimization

2、Optimize – Organics (Volatile)

Crystallization grade organics (volatile) for formulating screens or optimization

3、Optimize – Organics (Non-Volatile)

Crystallization grade organics (non-volatile) for formulating screens or optimization

4、Optimize – Salts

Crystallization grade salts for formulating screens or optimization

5、Optimize – Buffers

Crystallization grade buffers for formulating screens or optimization

6、Optimize – Solubilizing Agents (NDSB)

Crystallization grade non detergent sulfobetaines

7、Optimize – Reducing Agent

TCEP Hydrochloride

8、Optimize – Monoolein

LCP – Lipidic Cubic Phase

9、Optimize – Cryoprotectants

For cryoprotection of crystals

10、Optimize – Oils for Crystallization

Microbatch crystallization & oil layering

11、Optimize – Izit Crystal Dye

Izit™ color dye to differentiate protein crystals from salt crystals

Hampton 蛋白结晶向导管Wizard Tube

Wizard Tube 
The Wizard™ Classic line of random sparse matrix screens is designed to increase your probability of producing crystals during the coarse screening phase when crystallizing biological macromolecules (proteins, nucleic acids, peptides, and combinations thereof).
The Wizard Classic reagents are proven to be a highly effective starting point in the screening of biological macromolecules. The Wizard Classic formulations include a large range of crystallants, buffers, and salts covering a broad range of crystallization space at pH levels from pH 4.5 to pH 10.5.Choose from Wizard Classic 1, 2, 3, or 4 non-overlapping formulations in matrix blocks or tubes.

图片

Wizard Cubic LCP
The tools in the Wizard Cubic LCP (lipidic cubic phase) Kit enable researchers to prepare LCP-type crystallizations by hand. Ideal for low-protein experiments: effective protein volume for a single crystallization experiment is about 80 nanoliters. Wizard Cubic LCP Kit tools work especially well when traditional methods have failed to yield crystals. Lipidic cubic phase has worked well for the crystallization of 7TM membrane proteins (proteins with seven transmembrane helices). Four out of six GPCRs (G-protein coupled receptors), an important membrane protein class, and several microbial 7TM proteins have been crystallized using the LCP approach.

图片

CRYO (I、II)
The Wizard Cryo™ line of random sparse matrix screens is designed for scientists who want to avoid the additional step of optimizing a cryoprotectant condition. Every Wizard Cryo formulation flash-freezes to a clear, amorphous glass in liquid nitrogen or in a cryo-stream at 100K. Crystals can be frozen directly from their growth drops, avoiding the additional step of pre-equilibration with an artificial cryo-condition that can damage the crystal. Eleven different cryocrystallants and sparing use of glycerol ensures a broad sampling of possible cryo conditions. Choose from Wizard Cryo 1 or 2 formulations in tubes or Wizard Cryo 1 and 2 together in a 96-well matrix block.

图片

Hampton PEGRx 聚乙二醇结晶试剂盒

Hampton PEGRx

PEGRx 1 • PEGRx 2 • PEGRx HT

Applications   主要是调节样本的PH值

  Primary and secondary, polymer and pH based crystallization screen for biological macromolecules

PEGRx is a primary and secondary, polymer and pH based crystallization screen developed at Hampton Research. It is designed to evaluate polymer based crystallization reagents and pH in low (PEGRx 1) to medium ionic strength (PEGRx 2). It is also designed for use as a secondary or optimization screen to follow the Hampton Research Index screen when polymer based reagents produce hits and interesting solubility leads.PEGRx 1 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a low ionic strength environment versus a wide range of pH. Polymer reagents include Polyethylene glycols, Polyethylene glycol monomethyl ethers, and Jeffamines®. The molecular weight range between 200 and 20,000 is evaluated in a low ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and low ionic strength.

PEGRx 2 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a medium ionic strength environment in the presence of additives, salts, volatile organics and polyols versus a wide range of pH. Polymer reagents include Polyethylene glycols and Polyethylene glycol monomethyl ethers. The polymer molecular weight range between 200 and 20,000 is evaluated in a medium ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and secondary reagents which include additives, salts, volatile organics and polyols.上海金畔生物作为Hampton蛋白结晶产品的代理商,竭诚为您服务,欢迎新老客户咨询。PEGRx 1 contains 48 unique reagents, 10 ml each.

PEGRx 2 contains 48 unique reagents, 10 ml each

PEGRx HT contains the 96 PEGRx 1 and PEGRx 2 reagent in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

PEGRx 1 • PEGRx 2 • PEGRx HT

PEGRx

PEGRx 1 • PEGRx 2 • PEGRx HT

DesR crystals grown using Hampton Research PEGRx, Jin Ho Moon, Korea University.

CAT NO NAME DESCRIPTION
HR2-082 PEGRx 1 10 ml, tube format
HR2-084 PEGRx 2 10 ml, tube format
HR2-086 PEGRx HT 1 ml, Deep Well block format

Hampton 膜蛋白结晶试剂 MembFac • Crystal Screen Lite • MembFac HT

Hampton  MembFac • Crystal Screen Lite • MembFac HT

Applications

Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility   膜蛋白和样品溶解度有限样品

Features

  Membrane protein sparse matrix screens

  Sparse matrix formula efficiently samples salts, polymers, organics and pH

  pH range 4.6 – 8.5

  Formulated for use with detergents

   Tube or Deep Well block format

   Crystal Screen Lite features a lower ionic strength than the original Crystal Screen

Description
MembFac is a highly effective sparse matrix screen specifically designed as a preliminary screen for the crystallization of membrane proteins. MembFac is based upon the highly effective biased sparse matrix methodology. The MembFac screen matrix is optimized for the crystallization of membrane proteins.

MembFac contains 48 unique reagent formulations. With this set of 48 conditions, numerous chemicals and chemical combinations are utilized, allowing one to evaluate a large variety of potential crystallization conditions. MembFac covers a broad pH range between 4 and 9.

The Rationale
The basic rationale of MembFac is to perform a sparse matrix screen while changing the detergent dimension because detergents play an important factor in the crystallization of membrane proteins. A MembFac screen is to be completed for each detergent. Detergent screens are available separately.

The Method
The membrane protein of interest is first isolated in the detergent which gives the highest stability and activity. The protein concentration should be approximately 10 to 20 mg/ml and the detergent concentration should be only slightly above the CMC. The sample is then set against MembFac, using all 48 solutions plus the screening detergent(s) of choice. The screening detergent concentration should be approximately one to three times the CMC. Hence, for each detergent screened, one would utilize approximately 1 to 2 mg of sample.

The MembFac protocol was used to crystallize membrane proteins including SQR, fumerate: quinone oxireductase, LHII, and LHCII.

Crystal Screen Lite is a sparse matrix of trial crystallization reagent conditions based upon the Crystal Screen™.3 The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and non-volatile organics).2Crystal Screen Lite differs from the original Crystal Screen kit in that the primary precipitant reagents are one-half the concentration of that used in the original Crystal Screen formulation. The secondary salts, ions, and buffers remain at the original Crystal Screen concentration. Reducing the primary concentration of the primary precipitant results in a screen which is “more gentle” on the sample and typically produces much less precipitate conditions than the original Crystal Screen.

MembFac contains 48 unique reagents, 10 ml each.

MembFac HT contains 1 ml each of all 48 reagents from MembFac and reagents 1-48 from Crystal Screen Lite in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

CAT NO NAME DESCRIPTION
HR2-114 MembFac 10 ml, tube format
HR2-128 Crystal Screen Lite 10 ml, tube format
HR2-137 MembFac HT 1 ml, Deep Well block format