Hampton 蛋白结晶删选试剂– Index HT

Hampton 蛋白结晶删选试剂– Index HT

Applications

Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules

多样结晶试剂系统删选试剂盒,用于蛋白质,复合物,肽,核酸,和水溶性的小分子。

Features

 Developed at Hampton Research

Screens classic, contemporary, & modern crystallization reagents

Samples pH 3 to 9

Compatible with microbatch, vapor diffusion, & liquid diffusion methods

Specially formulated reagent zones:

1 .Traditional salts versus pH

2.Neutralized organic acids

3.High [salt] with low [polymer]

4.High [polymer] with low [salt]

5.Low ionic strength versus pH

6.PEG & Salt versus pH

7.PEG & Salt

 Tube or Deep Well block format

Description
Index is designed as a 96 reagent crystallization screen that combines the strategies of the grid, sparse matrix, and incomplete factorial screening with traditional, contemporary, and new crystallization reagent systems into a highly effective and efficient format.

Index, as the name implies, efficiently samples a series of specially formulated reagent zones to identify which reagent class or classes and pH are effective in producing crystals or limiting sample solubility. Results from Index can be used to design optimization experiments and to identify follow on screens by reagent class. For example, positive results with salt based reagents in Index may be followed up with further screening using SaltRx or Grid Screen Salt HT. Success with polymer based reagents in Index may be followed up with further screening using PEGRx or PEG/Ion.

Index utilizes a broad, yet refined portfolio of crystallization reagent systems. These include the following: (1) traditional salts such as Ammonium sulfate and Sodium chloride versus pH; (2) neutralized organic acids such as Sodium malonate and Tacsimate; (3) High salt concentration mixed with low polymer concentration as well as high polymer concentration mixed with low salt concentration and; (4) Low ionic strength using polymers such as PEG, MPD, Pentaerythritols versus pH. These reagent systems are formulated across a sparse matrix and incomplete factorial of concentration ranges, sampling a pH range of 3 to 9.

Index contains 96 unique reagents, 10 ml each.

Index HT contains 96 unique reagents in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Measured pH range of kit is 3 to 9 at 25°C
Average measured pH of kit is 6.8 at 25°C
Median measured pH of kit is 6.9 at 25°C

Index • Index HT

Index • Index HT

Apo Kinase crystal. Initial hit from the Hampton Research Index Screen. Annie Hassell, Glaxo Smithkline.

Index • Index HT

Crystal of a heme-based sensor from Thermoanaerobacter Tengcongensis grown using the Hampton Research Index screen. Patricia Pellicena & John Kuriyan at University of California, Berkeley, USA.

CAT NO NAME DESCRIPTION
HR2-144 Index 10 ml, tube format
HR2-134 Index HT 1 ml, Deep Well block format

Hampton 蛋白结晶试剂–Crystal Screen

Hampton 蛋白结晶试剂–Crystal Screen

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Applications

 Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules

 Sparse matrix additive screen

Features

 The original sparse matrix screen

 Sparse matrix formula efficiently samples salts, polymers, organics, & pH

 Proven effective with more than 1,000 biological macromolecules

 Tube or Deep Well block format

Description
The Crystal Screen and Crystal Screen 2 reagent kits are designed to provide a highly effective and rapid screening method for the crystallization of macromolecules. The screens are simple and practical for finding initial crystallization conditions. The initial crystallization conditions for more than 1,000 proteins, peptides, oligonucleotides, and small molecules have been determined using Crystal Screen.

A highly effective approach to overcome the exhaustive search for suitable crystallization conditions is the use of a sparse matrix method of trial conditions that is biased and selected from known crystallization conditions for macromolecules. The formulation utilized in Crystal Screen and Crystal Screen 2 evaluates 96 unique mixtures of pH, salts, polymers and organics, and their ability to promote crystal growth.

Crystal Screen contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by Jancarik and Kim in 1991.

Crystal Screen 2, an extension of Crystal Screen, contains 48 unique reagents, 10 ml each and is based on the formulation first described by Cudney et al in 1994.

Crystal Screen HT contains 1 ml each of reagents 1-48 from Crystal Screen and all 48 reagents from Crystal Screen 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Crystals of a carbohydrate processing enzyme grown using Hampton Research Crystal Screen. Shirley Roberts, York Structural Biology Laboratory, York, United Kingdom.
Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Crystal of zRFP574 red fluorescent protein grown using Hampton Research Crystal Screen 2. Pletneva et al. Acta Cryst. D62, 527-532 (2006). Nadya, Russian Academy of Science, Moscow, Russia.

CAT NO NAME DESCRIPTION
HR2-110 Crystal Screen 10 ml, tube format
HR2-112 Crystal Screen 2 10 ml, tube format
HR2-130 Crystal Screen HT 1 ml, Deep Well block format

Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate

Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate

美国Hampton Research公司位于加利福尼亚州,是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材,同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线,不仅为全世界晶体研究人员的工作带来了便捷,而且也受益于科研人员的回馈不断发展自己,目前已成为晶体研究领域最受信赖的品牌之一。

Hampton research主要产品:

Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液

Optimize Reagents 结晶级别盐类,聚合物,有机溶液或缓冲液

Crystallization Plates, Hardware & Accessories 结晶板(悬滴法和坐滴法,微量透析,微配液,自由界面扩散,凝胶以及无容器悬浮法)及其他配件

Cryocrystallography 低温晶体学耗材如 Crystalcap system,cryoloops

Goniometer Heads&Supplies 测角器头和配件

Protein Crystallization Standards 结晶级别标准蛋白

Custom Shop Crystallization Reagents 定制试剂盒和结晶溶液内的单一组分

StockOptions Kits 内含盐类,缓冲液和冷冻液的简便型试剂盒

Tools,Seeding&Resin 结晶用探针,结晶笔,seeding法结晶工具

Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂

Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate

HDP Hanging Drop Crystallization Plate (Swissci)

HDP Hanging Drop Plate

HDP Hanging Drop Crystallization Plate (Swissci)

Single Well with Tool

HDP Hanging Drop Crystallization Plate (Swissci)

Hanging Drop Wells

Applications—Hanging drop crystallization

Features

  Made from optically superior polymer (UVP)

  Allen key novel crystal plug closure system

  Wells are micro-numbered

Description  描述:
The Swissci Hanging Drop Crystallization Plate is a breakthrough for protein crystallization in a 96-well format. For the first time individual wells can be identified and removed without any disturbance to the growing crystals inside the plate.Description

The plate has been developed by Swissci AG together with leading Crystallographers. It is the result of many years of experience in successful robotic high-throughput crystallization.

The Hanging Drop Crystallization Plate offers unique properties that make it ideal for both nanoliter crystallization screening and microliter optimization alike. Made from optically superior polymer (UVP) and with a new design of the wells, the plate allows easy crystal viewing and retrieval. The system is fully covered by design and patent protection.

The Advantages of the Hanging Drop Crystallization Plate

Easy Crystal Retrieval
Raised wide wells make the crystal mounting especially easy. Allen key system allows for smooth removal and subsequent microscopic investigation / X-ray with the novel crystal plug closure all included in the system.

Easy Viewing
The wells are wide conical and have a lens effect for perfect illumination. The micro-numbering ensures you will never loose the location under the microscope.

The optically superior polymer is UV transmissible and can be used to differentiate between salt and protein crystals.

Integral Sealing
Wide partition walls between each well provides a high surface area for very good sealing with the advanced quality sealing tape. The sealing tape is integrated into the kit so there is no need to purchase additional parts prior to starting a procedure.

Wide Range of Volumes
Typical volumes are 50 – 200 µl of reservoir and 10 nanoliter – 5 microliter drop size.

SBS Standard
The complete plate is designed to the 96-well SBS standard for all common holders.

CAT NO NAME DESCRIPTION
HR3-187 Hanging Drop Crystallization Plate (Swissci) 10 plate case

Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate 相关产品:

96 Well Hanging Drop Vapor Diffusion Plate
Hanging drop crystallization
ARI Hanging Drop Seals for 96 Well Plates
ARI Hanging Drop Seals for 96 Well Plates
CrystalQuick 96 Well Sitting Drop Plate (Greiner)
Sitting drop crystallization
Detergent Screen HT
96 detergents Deep Well block format
Glass Cover Slide Gizmo Dispenser
Cover Slide Dispenser
Greiner ComboPlate and CrystalBridge
Hanging and sitting drop crystallization
HDP Hanging Drop Crystallization Plate (Swissci)
Hanging Drop Crystallization
Linbro Plate
Hanging drop crystallization
ProCrystal
Protein Crystallization Covers for Hanging Drop Vapor Diffusion
Protein Methods
Daniel M. Bollag, Michael D. Rozycki, & Stuart J. Edelstein
Solubility & Stability Screen
Identify protein specific additives that promote solubility and stability
UVP Hanging Drop Plate Seal (Swissci)
Swissci UVP Hanging Drop Plate Seal
VDX Plate with sealant
Hanging drop crystallization
VDX Plate without sealant
Hanging drop crystallization
VDX48 Plate with sealant
Hanging drop crystallization
VDXm Plate without sealant
Hanging drop crystallization
VDXm Plate with sealant
Hanging drop crystallization

 

Hampton 蛋白结晶优化试剂 Optimization Screens

Hampton 蛋白结晶优化试剂 Optimization Screens

1、Solubility & Stability Screen  蛋白特异性试剂优化溶解性和稳定性

Protein specific agents for optimization of solubility & stability  

2、Solubility & Stability Screen 2   离子缓冲液

Buffer, pH & ionic strength screen for optimization of solubility & stability

3、Slice pH    PH缓冲液

pH & buffer screen for optimization of crystals or sample solubility & stability

4、Additive Screen  96孔板添加剂试剂盒

96 compound screen for optimization of crystal size & quality

5、Silver Bullets • Silver Bullets Bio

Small molecule additive screen for optimization of crystal size & quality

6、Detergent Screen

Detergent screen for optimization of crystals or sample solubility & stability

7、Ionic Liquid Screen

Ionic liquid additive screen for optimization of crystal size & quality

8、StockOptions Buffer Kits

Titrated buffer sets for optimization of crystals or sample solubility & stability

9、StockOptions Polymer

n of crystal size & quality

10、StockOptions Salt

Salt reagent kit for optimization of crystal size & qualit

11、Polymer reagent kit for optimizatioy

Proti-Ace

In situ proteolysis kit protein for crystallization screening & optimization

12、Reductive Alkylation

Reductive alkylation kit for crystallization screening & optimization

13、Silica Hydrogel Kit

pH neutral silica hydrogel matrix crystallization

14、Low Melting Agarose

Agarose gel matrix crystallization

15、CryoPro

Water soluble cryoprotectant reagent set

16、Heavy Atom Screens

Heavy atom kits for multiple isomorphous replacement

17、I3C Phasing Kit

Produce heavy atom derivative of biological macromolecules for phasing

Hampton蛋白结晶工具及仪器

Hampton蛋白结晶工具及仪器

CryoTong™ – Long
ApplicationsCrystal transfer under cryo temperature
FeaturesArtery clamp style closure30 seconds of cryo temperature180 mm length
Description
The CryoTong is a tool used to manually transfer a crystal mounted on a CrystalCap™ from liquid nitrogen to a magnetic base in a goniometer head positioned in a cryogenic stream, and then back to liquid nitrogen. The one-piece, compact CryoTong is available in two lengths. The long CryoTong is approximately 180 mm in overall length. The artery clamp style maintains the CryoTong in the closed position until the clamp is squeezed, which opens the opposing heads. The heads are non-magnetic stainless steel. The handle is magnetic stainless steel. The inside of the head is machined to closely surround the CrystalCap with loop and crystal in place. A small retaining lip is machined into the lower portion of the head to prevent the CrystalCap from slipping out of the CryoTong when the tool is in the closed position. The CryoTong can maintain the temperature of the crystal at -160°C for up to 30 seconds during room temperature crystal transfers. Choose the proper CryoTong size based upon the pin length required by the configuration of the x-ray data collection hardware used. The 18 mm CryoTong (HR5-112) is to be used with the CrystalCap HT and any other CrystalCap configured for 18 mm.HR5-114 Long CryoTong 18 mm, 180° features the head in alignment with the handle of the CryoTong.

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Vial Clamp™ – Straight
ApplicationsVial support and manipulation
FeaturesStraight end tipHemostat style closureDescription
The Vial Clamp™ – Straight is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems™ straight when the clamp is closed. The clamp can be locked using the hemostat style closure. This clamp makes it easy to dip the storage vial into liquid nitrogen for crystal storage. The complete length of the clamp is straight, with an overall length of 195 mm. When the vial is placed in the clamp, the length of it is positioned perpendicular, 90° to the length of the clamp.
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CrystalWand™ Magnetic
ApplicationsCrystalCap Magnetic & CrystalCap Copper Magnetic handling toolDescription
The CrystalWand Magnetic is designed to be used exclusively with the CrystalCap Magnetic, CrystalCap Copper Magnetic, and CrystalCap HT systems during transfer of the caps from the vial to the goniometer head and from the goniometer to the vial. The 6 1/2” (165 mm) long chrome plated steel wand features a plastic housing enclosing a spring tensioned plunger that when depressed, moves a non-magnetic steel platform away from the magnet housed in the end of the wand. This causes the steel CrystalCap to detach from the wand and be placed readily into the vial or the CryoTong™. When the platform is retracted, the wand securely holds the steel CrystalCap Magnetic. The CrystalWand Magnetic 45° (McMiken Tool) offers the same functionality but is bent at a 45° angle at approximately 2.5 cm from the magnet end.HR4-729 CrystalWand Magnetic, StraightHR4-315 CrystalWand Magnetic, 45° (McMiken Tool)

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Vial Clamp™ – Curved
ApplicationsVial support & manipulation
FeaturesCurved end tipHemostat style closureDescription
The Vial Clamp – Curved is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems at an angle when the clamp is closed. The clamp can be locked using the hemostat style closure. The end of the clamp, where the vial is held, is curved at either a 45°/135° or 110°/ 70° angle. When the vial is placed in the clamp, the length of it is positioned to the clamp at either of those same angles. The overall length of each clamp is the same, 195 mm.HR4-671 Vial Clamp – Curved 45°/135°HR4-672 Vial Clamp – Curved 110°/70°

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CrystalCap™ Holder
pplicationsCrystalCap support inside stainless steel dewars
FeaturesAdjustable heightSupports up to 8 CrystalCaps15 ml vial position for liquid propane500 and 1,000 ml versionsDescription
CrystalCap Holders are convenient stands for holding all CrystalCap™ systems in liquid nitrogen dewars during crystal cryogenic procedures. Available in two sizes. Both holders feature spring-clips to secure the CrystalCap Vials in the holder. Both stands have 3 inches of adjustable Z (height) which allows one to raise or lower the CrystalCaps to the appropriate height, depending upon the liquid nitrogen level in the dewar. Each holder is supplied with a socket driver for height adjustment.500 ml version holds five CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 500 ml dewar. 1,000 ml version holds eight CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 1,000 ml dewar.HR4-707 CrystalCap Holder – 500 ml
HR4-706  Replacement Height Adjustment Tool

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Hampton 0.1毫米50号玻璃毛细管

Hampton 0.1毫米50号玻璃毛细管

货号:HR6-104
包装:25 pack

  • 英文名:0.1 mm Glass Number 50 Capillary
  • 品牌:Hampton
Hampton 0.1毫米50号玻璃毛细管

Hampton代理–金畔生物

硼硅玻璃50号毛细管壁厚0.01毫米,全长80毫米+ / – 5毫米。提供外径从0.1毫米到2毫米的毛细管,用于X射线数据收集,晶体密度测量和晶体生长实验。毛细管可以用蜡,环氧树脂等密封材料封闭,防止水分和气体。

应用

X射线数据采集

液-液扩散结晶

凝胶针状结晶

特征

50号硼硅玻璃

壁厚 10微米

描述

硼硅玻璃50号毛细管一端呈漏斗形,另一端封闭,壁厚0.01毫米,全长80毫米+/-5毫米,外径从0.1毫米到2毫米。可嵌入,盛放,储存小分子和生物大分子晶体用于X射线数据收集,晶体密度测量和晶体生长实验。毛细管可以用蜡,环氧树脂和其它密封材料密封,防止水分和气体。

请参照“线性吸收系数µcm-1表“,以决定适合的玻璃/石英玻璃毛细管。本表展示的是X射线数据收集时的辐射吸收量。

0.1mm至2.0mm毛细管开口端的基础尺寸为:3.0+/-0.15mm外径 x 0.13+/-0.10 mm壁厚,直径为在毛细管封闭端40毫米处的测量值。

 

货号

产品说明

公差

HR6-104

Size: 0.1 mm – 25 pack

0.1 mm -0.075 / +0.075 mm

HR6-106

Size: 0.2 mm – 25 pack

0.2 mm -0.050 / +0.050 mm

HR6-108

Size: 0.3 mm – 25 pack

0.3 mm -0.050 / +0.100 mm

HR6-110

Size: 0.4 mm – 25 pack

0.4 mm -0.100 / +0.100 mm

HR6-112

Size: 0.5 mm – 25 pack

0.5 mm -0.100 / +0.100 mm

HR6-114

Size: 0.6 mm – 25 pack

0.6 mm -0.100 / +0.100 mm

HR6-116

Size: 0.7 mm – 25 pack

0.7 mm -0.150 / +0.150 mm

HR6-118

Size: 0.8 mm – 25 pack

0.8 mm -0.150 / +0.150 mm

HR6-120

Size: 0.9 mm – 25 pack

0.9 mm -0.150 / +0.150 mm

HR6-122

Size: 1.0 mm – 25 pack

1.0 mm -0.150 / +0.250 mm

HR6-124

Size: 1.5 mm – 25 pack

1.5 mm -0.250 / +0.250 mm

HR6-126

Size: 2.0 mm – 25 pack

2.0 mm -0.250 / +0.250 mm

Hampton Optimize Reagents

Hampton Optimize Reagents

1、Optimize – Polymers

Crystallization grade polymers for formulating screens or optimization

2、Optimize – Organics (Volatile)

Crystallization grade organics (volatile) for formulating screens or optimization

3、Optimize – Organics (Non-Volatile)

Crystallization grade organics (non-volatile) for formulating screens or optimization

4、Optimize – Salts

Crystallization grade salts for formulating screens or optimization

5、Optimize – Buffers

Crystallization grade buffers for formulating screens or optimization

6、Optimize – Solubilizing Agents (NDSB)

Crystallization grade non detergent sulfobetaines

7、Optimize – Reducing Agent

TCEP Hydrochloride

8、Optimize – Monoolein

LCP – Lipidic Cubic Phase

9、Optimize – Cryoprotectants

For cryoprotection of crystals

10、Optimize – Oils for Crystallization

Microbatch crystallization & oil layering

11、Optimize – Izit Crystal Dye

Izit™ color dye to differentiate protein crystals from salt crystals

Hampton Natrix • Natrix 2 • Natrix HT

Hampton Natrix • Natrix 2 • Natrix HT

Applications

  Primary biased sparse matrix crystallization screen for nucleic acids & protein/nucleic acid complexes  主要用于核酸和蛋白质/核酸复合物的删选

Features

Nucleic acid sparse matrix screen
Sparse matrix formulation efficiently samples salts, polyols, polymers, organics, additives & pH
pH range 5.6 – 8.5
Tube or Deep Well block format
 Description
Natrix, Natrix 2 and Natrix HT are based upon published reagent formulations for the crystallization of nucleic acids and protein-nucleic acid complexes. A variety of hammerhead ribozymes and other ribozymes, RNAs, DNAs, RNA-drug complexes, and RNA-protein complexes have been crystallized using the Natrix protocols.By using sparse matrix sampling technology, The Natrix kits allow one to quickly test wide ranges of pH, salts, and precipitants using a very small sample of nucleic acid.

Natrix screens are unique in that rather than relying solely on the traditional nucleic acid precipitant MPD, Natrix screens also utilize Polyethylene glycols (PEGs) in a variety of molecular weights (200, 400, 4,000, 8,000) as well as 2-Propanol, Polyethylene glycol monomethyl ether (PEG MME), and 1,6-Hexanediol. Many of the polymeric and low molecular weight organic precipitants are combined with various monovalent salts as precipitating agents. This combination of salts and low molecular weight organics and polyalcohols, as well as the utilization of varying chain length PEGs, has proven to be a successful combination for producing nucleic acid and protein-nucleic acid complex crystals.

Natrix contains 48 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by William Scott in 1995.

Natrix 2, an extension of Natrix, contains 48 unique reagents, 10 ml each. Natrix 2 is a biased sparse matrix screen based on extracting patterns from crystallization data as well as reagent formulations first described by Berger et al in 1996.

Natrix HT contains 1 ml of each reagent from Natrix and Natrix 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
View Full Size
Natrix • Natrix 2 • Natrix HT

 

CAT NO NAME DESCRIPTION
HR2-116 Natrix 10 ml, tube format
HR2-117 Natrix 2 10 ml, tube format
HR2-131 Natrix HT 1 ml, Deep Well block format

Hampton 银弹 Silver Bullets

Hampton 银弹 Silver Bullets

Applications

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Developed at Hampton Research
Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

Description
Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3

Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3

X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.

Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests

The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:

•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal

The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ºC, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.

Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.

Silver Bullets reagent volume is 0.5 ml (each well).

ThermoFluor is a registered trademark of Johnson & Johnson.

Hampton  Silver Bullets Bio

Silver Bullets • Silver Bullets Bio

Silver Bullets • Silver Bullets Bio

上海金畔生物作为Hampton蛋白结晶产品的代理商,竭诚为您服务,欢迎新老客户咨询。

Applications 应用

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

 

Developed at Hampton Research
Two sets of 96 reagents, composed of more than 1,090 chemicals, of which more than 400 are uniqu

Please note, the Silver Bullets Bio Screen is temporarily unavailable as a new formulation is in development

 

Hampton Siliconized Glass Cover Slides

Hampton Siliconized Glass Cover Slides

ApplicationsHanging, sitting or sandwich drop crystallization
FeaturesHigh purity of glassSiliconized, hydrophobic glass surface0.22 mm & 0.96 mm glass thicknessThicker glass slides preserve temperature longer12 mm, 18 mm, & 22 mm glass diameterCircle & squareUV Compatible (>85% transmission)
12 x 0.22 mm Circle
18 x 0.22 mm Circle
22 x 0.22 mm Circle & Square

Description
Siliconized glass cover slides allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution. These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use Dow Corning Vacuum Grease (HR3-510), Dow Corning 7 Release Compound (HR3-508), or immersion oil to seal cover slide to plate. Available in 12, 18 or 22 mm diameter circles and 22 mm diameter squares. Available in 0.22 mm or 0.96 mm glass thickness. The 0.96 mm thick slides are virtually unbreakable during crystallization handling.

The following slides are UV transmissable (>85%):
HR3-277 and HR3-279 – 12 mm x 0.22 mm Siliconized circle cover slides
HR3-231 and HR3-233 – 22 mm x 0.22 mm Siliconized circle cover slides
HR3-239 and HR3-241 – 18 mm x 0.22 mm Siliconized circle cover slides
HR3-215 and HR3-217 – 22 mm x 0.22 mm Siliconized square cover slides

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Plain Glass Cover Slides
ApplicationsHanging, sitting or sandwich drop crystallization
Features
0.22 mm glass thickness
12 mm glass diameter
Hydrophilic glass surface
Circle & squareDescription
Plain, non-siliconized, hydrophilic glass cover slides. Useful for hanging drop, sitting drop, and sandwich drop vapor diffusion crystallization methods. The hydrophilic surface creates a drop which is more flat than a drop on a siliconized surface. This offers enhanced imaging for very small drops. Use Dow Corning® Vacuum Grease (HR3-510), Dow Corning® 7 Release Compound (HR3-508), or immersion oil to seal cover slide to plate.

HR3-209T 12 mm Plain circle cover slides for Tecan robot 5 oz case (~2,400 slides)

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HR3-209T 12 mm Plain circle cover slides for Tecan robot 5 oz case (~2,400 slides)
PEN-VAC Kit
ApplicationsHolding and manipulating small, flat surfaces such as glass cover slides
FeaturesSelf-contained vacuumNo power neededCompactDescription
PEN-VAC is a great tool for handling small, flat surfaced objects such as plain and siliconized cover slides, as well as plastic slides. Simply depress the side button, touch the tip to the object to be lifted and release the button. PEN-VAC creates a totally self-contained vacuum that lifts up to 50 grams for up to one minute. Less than an ounce, the lightweight aluminum body fits in a pocket. No power supply needed. The kit includes one PEN-VAC with 5 3/4″ blue body, (3) angled and (3) straight probes with (2) each 1/8”, 1/4”, 3/8” diameter blue silicone vacuum cups . It is packaged in a clear plastic storage case.

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HR3-251  PEN-VAC Kit