低温泡沫杜瓦瓶 Spearlab Cryogenic Foam Dewars

低温泡沫杜瓦瓶 Spearlab Cryogenic Foam Dewars
Spearlab Cryogenic Foam Dewars
Spearlab Dewars
Spearlab Cryogenic Foam Dewars
HR4-662 Cryogenic Foam Dewar (500 ml) – Small  500ml规格
Spearlab Cryogenic Foam Dewars
HR4-675 – Spearlab Cryogenic Foam Dewar (2 liter)  2L规格
低温泡沫杜瓦瓶 Spearlab Cryogenic Foam Dewars

Applications 应用

    Cryocrystallography 蛋白结晶学

Features

  Each dewar is supplied with a lid  每个杜瓦瓶都配有盖子

  Proprietary foam construction (USPTO # 7,971,744)

  Mini (130 ml), Small (500 ml), Standard (800 ml), Large (1400 ml) and Tall (1800 ml)

   Reduced ice formation on dewar lip

  Easier to dry

   Foam construction reduces slipping

Description
Spearlab FD-130 Cryogenic Foam Dewar (130 ml) – Mini : Small, hexagonal outside, hand held foam dewar, maximum volume 175 ml. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 2 inches in diameter by 3.5 inches deep, so that it easily holds 130 ml and a maximum volume of 175 ml of liquid nitrogen. The HR4-676 has an overall height of approximately 4.5 inches without cover and the width is 3.5 inches at the top and 3.75 inches on the bottom (measured on the flats of the hexagon). Circular cover is approximately 0.5 inches tall x 3.5 inches diameter.

Spearlab Cryogenic Foam Dewar (500 ml) – Small: The small foam dewar shape is circular, with a protruding handle, so that it resembles a large teardrop. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 4.5 inches in diameter by 2.8 inches deep, so that it easily holds 500 ml of liquid nitrogen.

Spearlab FD-800 Cryogenic Foam Dewar (800 ml) – Standard: The standard foam dewar shape is circular, with a protruding handle, so that it resembles a large teardrop. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 5.8 inches in diameter by 2.8 inches deep, so that it easily holds 800 ml of liquid nitrogen.

Spearlab FD-1400 Cryogenic Foam Dewar (1400 ml) – Large: The large foam dewar shape is circular, with a protruding handle, so that it resembles a large teardrop. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 6.3 inches in diameter by 3.8 inches deep, so that it easily holds 1400 ml of liquid nitrogen. Overall height is approximately 6 inches without cover and the width is 9 inches on the short axis and 11 inches on the long axis. Cover approximately 0.5 inches tall x 9 inches diameter.

Spearlab FD-1800 Cryogenic Foam Dewar (1800 ml) – Tall: The tall foam dewar shape is a tapered octagon on the outside with a cylindrical interior. The tapered octagon features a wide, stable base. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 3.5 inches in diameter by 12.5 inches deep, so that it holds approximately 1,800 ml of liquid nitrogen. The outside dimensions of the tall dewar are 15.25 inches tall with a 6.25 inches wide top and a 7.75 inches wide base (measured on the flats of the octagon). The cover is 6.5 inches in diameter and 0.5 inches thick.

The patented (USPTO # 7,971,744) design of the Spearlab Cryogenic Foam Dewar makes it easy to handle and safer to use than a traditional low profile glass dewar. Also, because of its lower thermal mass, a foam vessel will cause less boil off when it is filled with liquid nitrogen. Additionally, the dewar will accumulate less frost during regular use. The end result is that less liquid nitrogen is consumed.

The dewars are made from cross-linked polyethylene foam. A density of 4 pounds per cubic foot is used for the 130 ml and 800 ml dewars. A density of 2 pounds per foot is used for the 1400 ml and 2 liter dewars. The higher density foam has significantly higher mechanical strength and stiffness. The lower density foam has slightly lower thermal conductivity. Like most lightweight insulation materials, the thermal conductivity of the foam is predominantly determined by the thermal conductivity of air, which eventually permeates the pores of the foam material.

CAT NO NAME DESCRIPTION
HR4-676 Cryogenic Foam Dewar (130 ml) – Mini each
HR4-662 Cryogenic Foam Dewar (500 ml) – Small each
HR4-673 Cryogenic Foam Dewar (800 ml) – Standard each
HR4-674 Cryogenic Foam Dewar (1400 ml) – Large each
HR4-675 Cryogenic Foam Dewar (2 liter) – Tall each

Hampton CrystalEX Second Generation (Corning)

Hampton CrystalEX Second Generation (Corning)

Crystal<i>EX</i> Second Generation (Corning)

CrystalEx Plates

Applications

   Sitting drop crystallization

Features

96 well sitting drop plate
Round and conical flat bottom drop well shapes
1, 2 or 4 microliter drop wells
1, 3 or 5 drop wells per reservoir
Hydrophilicity treated or untreated
PZero or COC plate material–UV imaging compatible

Description

The second generation of Corning® 96 Well, sitting drop format plates are built to SBS specifications, making them well suited for high throughput crystallization and are fully compatible with robotic equipment. The plates are available in several different configurations with varying drop well shapes, plate materials, and number of drop wells per reagent well. The basic plate design is one reagent well flanked by one or three drop wells, with SBS standard spacing between the centers of adjacent well clusters. One may choose a plate with small (1 µl), medium (2 µl), or large (4 µl) drop well volumes. The choice of round or conical flat well shapes are available. The PZero polymer provides for zero background polarization and is non-birefringent. PZero plates are not treated. The COC polymer offers high chemical resistance. Both types of plastic feature improved transparency. The reservoir numbers are embossed on each individual well for easy identification. Drop well locations conform to SBS standards for robotic handling. The low-volume reagent well saves on reagent costs. The plates can be sealed using Crystal Clear Sealing Film (HR3-609), 3 inch wide Crystal Clear Sealing Tape (HR4-506) or ClearSeal Film™ (HR4-521).

Corning has discontinued 3557 – special pricing and limited inventory available for this item only.

CAT NO NAME DESCRIPTION
HR8-135 Corning 3556 4µl round drop well, 1 drop well, COC, untreated – 10 plate case
HR8-134 Corning 3556 4 µl  round drop well, 1 drop well, COC, untreated – 40 plate case
HR8-137 Corning 3551 4 µl conical flat drop well, 1 drop well, COC, treated – 10 plate case
HR8-136 Corning 3551 4µl conical flat drop well, 1 drop well, COC, treated – 40 plate case
HR8-139 Corning 3552 2 µl round drop well, 3 drop well, COC, untreated – 10 plate case
HR8-138 Corning 3552 2µl  round drop well, 3 drop well, COC, untreated – 40 plate case
HR8-141 Corning 3553 2 µl conical flat drop well, 3 drop well, COC, untreated – 10 plate case
HR8-140 Corning 3553 2 µl conical flat drop well, 3 drop well, COC, untreated – 40 plate case
HR8-147 Corning 3550 1µll conical flat drop well, 3 drop well, COC, untreated – 10 plate case
HR8-146 Corning 3550 1µl conical flat drop well, 3 drop well, COC, untreated – 40 plate case
HR8-160 Corning 3557 1 µl conical flat drop well, 5 drop well, PZero – 10 plate case
HR8-158 Corning 3557 1 µl conical flat dro p well, 5 drop well, PZero – 40 plate case

Hampton Natrix • Natrix 2 • Natrix HT

Hampton Natrix • Natrix 2 • Natrix HT

Applications

  Primary biased sparse matrix crystallization screen for nucleic acids & protein/nucleic acid complexes  主要用于核酸和蛋白质/核酸复合物的删选

Features

Nucleic acid sparse matrix screen
Sparse matrix formulation efficiently samples salts, polyols, polymers, organics, additives & pH
pH range 5.6 – 8.5
Tube or Deep Well block format
 Description
Natrix, Natrix 2 and Natrix HT are based upon published reagent formulations for the crystallization of nucleic acids and protein-nucleic acid complexes. A variety of hammerhead ribozymes and other ribozymes, RNAs, DNAs, RNA-drug complexes, and RNA-protein complexes have been crystallized using the Natrix protocols.By using sparse matrix sampling technology, The Natrix kits allow one to quickly test wide ranges of pH, salts, and precipitants using a very small sample of nucleic acid.

Natrix screens are unique in that rather than relying solely on the traditional nucleic acid precipitant MPD, Natrix screens also utilize Polyethylene glycols (PEGs) in a variety of molecular weights (200, 400, 4,000, 8,000) as well as 2-Propanol, Polyethylene glycol monomethyl ether (PEG MME), and 1,6-Hexanediol. Many of the polymeric and low molecular weight organic precipitants are combined with various monovalent salts as precipitating agents. This combination of salts and low molecular weight organics and polyalcohols, as well as the utilization of varying chain length PEGs, has proven to be a successful combination for producing nucleic acid and protein-nucleic acid complex crystals.

Natrix contains 48 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by William Scott in 1995.

Natrix 2, an extension of Natrix, contains 48 unique reagents, 10 ml each. Natrix 2 is a biased sparse matrix screen based on extracting patterns from crystallization data as well as reagent formulations first described by Berger et al in 1996.

Natrix HT contains 1 ml of each reagent from Natrix and Natrix 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
View Full Size
Natrix • Natrix 2 • Natrix HT

 

CAT NO NAME DESCRIPTION
HR2-116 Natrix 10 ml, tube format
HR2-117 Natrix 2 10 ml, tube format
HR2-131 Natrix HT 1 ml, Deep Well block format

Hampton 96孔坐滴蛋白结晶板–Intelli-Plate

Hampton 96孔坐滴蛋白结晶板–Intelli-Plate

在生命科学领域,上海金畔生物为业界提供了丰富的实验室和生物医药生产研发的产品。庆祝上海金畔生物正式成为Hampton Intelli-Plate 96孔蛋白结晶板的签约代理商,在这里要感谢广大客户多年来对上海金畔生物科技有限公司的支持和厚爱,我们将一如既往的为广大客户带来Hampton Intelli-Plate 96孔蛋白结晶板高品质的产品和服务,欢迎广大新老客户来电咨询。

Intelli-Plate 96 (Art Robbins Instruments)

Applications

  Sitting Drop Crystallization   用于蛋白坐滴结晶

Features  特征:

SBS microplate footprint & standard well spacing
Optically clear wells provide for superior crystal imaging
Well layout makes crystal harvesting easier
UV transmissible and low birefringence
2 well versions available in small and large drop well or two identical shallow drop wells
3 well versions are ideal for optimizing protein concentration, additive screens, drop ratio and combinatorial experiments
Rounded reservoir corners to prevent reservoir (MPD) creep

Description

Art Robbins Instruments has developed the ideal family of plates for crystallography applications: the Intelli-Plate. All versions of the Intelli-Plate have the SBS microplate footprint and standard well spacing and are ideal for manual or automated processing.

The Intelli-Plate 96-2 Low Profile (Art Robbins 102-0001-10 Hampton Research HR3-116 and HR3-117) is a low profile version of the Intelli-Plate 96-2 LVR (Low Volume Reservoir). Reservoir volume: 100 µl max. Drop well volume: 4 µl and 10 µl max. UV transmissible and low birefringence.

The CrystalMation Intelli-Plate 96-3 low-profile crystallization plate (Art Robbins 102-0001-13 Hampton Research HR3-118 and HR3-119) is a low profile version of the Intelli-Plate 96-3 LVR (Low Volume Reservoir). The plate is designed for sitting drop vapor diffusion crystallization experiments. It is constructed from optically clear, UV-transmissible, chemically resistant plastic with superior low birefringence. The low-profile construction allows for higher density in plate storage and imaging systems, maximizing your investment and valuable space. The plate is designed to be a substantial improvement in existing low-profile plates, allowing for superior sealing due to thicker well design and a flat sealing surface. It has been built to the SBS (Society for Biomolecular Screening) standard dimensions with 8 vertical wells along the left side of the plate (A-H) and 12 horizontal wells along the top of the plate (1-12). It is compatible with automated instrumentation including the CrystalMation line of instruments from Rigaku. The plate features three identical sized sample drop wells per reservoir. The wells are concave depressions along the left side (Y-axis) of the plate and are located on the ledge above the adjacent, flat bottom reagent reservoir. Each well features a round bottom for easy crystal harvesting and can hold up to 1 µl of sample. The multiple well design allows for combinatorial experiments, complex co-crystallization experiments for ligand screening, or for drop concentration variation. The reagent reservoir is typically filled with 50 to 70 µl of reagent. Reservoir volume: 100 µl max. Drop well volume: 1 µl max. UV transmissible and low birefringence.

The Intelli-Plate 96-2 LVR (Art Robbins 102-0001-00 Hampton Research HR3-143 and HR3-145) is a Low Volume Reservoir version of the original Intelli-Plate. Reservoir volume: 100 µl max. Drop well volume: 1 µl max. UV transmissible and low birefringence.

The Intelli-Plate 96-3 LVR (Art Robbins 102-0001-03 Hampton Research HR3-183 and HR3-185) is a Low Volume Reservoir, 3 drop well version of the Intelli-Plate. Reservoir volume: 100 µl max. Typical reservoir volume 50 µl. Drop well volume: 1 µl max. UV transmissible and low birefringence.

The Intelli-Plate 96-2 Original crystallization plate (Art Robbins 102-0011-00 Hampton Research HR3-297 and HR3-299) is an optically clear plate for sitting drop vapor diffusion crystallization. All wells have standard 9 mm spacing to conform to SBS standards with 8 vertical wells along the left side of the plate (A-H) and 12 horizontal wells along the top of the plate (1-12). This plate features two locations for sample per reservoir. The sample drop locations are located along the left side of the reagent reservoir, along the Y-axis of the plate. The sample wells are concave depressions on the ledge above the adjacent, flat bottom reagent reservoir. One sample well is located above the second sample well. The top sample well can hold 10 µl or less of sample. The lower sample well can hold 4 µl or less of sample. The reagent reservoir is typically filled with 100 µl of reagent and is capable of holding up to 300 µl of reagent. The sidewalls separating adjacent wells or reservoirs are 0.9 and 1.0 mm thick in order to offer a larger area for sealing the plate and a separation of the reservoirs. This plate is rigid, with virtually no torsional flex and is designed for either manual or automated pipetting. UV transmissible and low birefringence.

The Intelli-Plate Flat Shelf (Art Robbins 102-0001-01 Hampton Research HR8-171 and HR8-172) crystallization plate is a flat drop shelf version of the original Intelli-Plate. Reservoir volume: 300 µl max. No drop well. UV transmissible and low birefringence.

The Intelli-Plate 96-2 Shallow Well (Art Robbins 102-0001-20 Hampton Research HR3-163 and HR3-164) has two identically sized 2 µL reaction wells and a 140 µL screen reservoir. Each of the two identical well features a round bottom shallow well design for easy crystal harvesting and is arranged in an 8 x 12 array. This plate has low birefringence and is UV compatible. This plate is also available in a low profile height (Art Robbins 102-0001-21).

The plates can be sealed using Crystal Clear Sealing Film (HR3-609), 3 inch wide Crystal Clear Sealing Tape (HR4-506) or ClearSeal Film™ (HR4-521).

The height of Intelli-Plate is 0.560 inches. The height of Intelli-Plate Low Profile is 0.315 inches (8.001 mm).

Intelli-Plate 96 (Art Robbins Instruments)

Intelli-Plate 96-3

CAT NO NAME DESCRIPTION
HR3-116 Intelli-Plate 96-2 Low Profile 20 plate case
HR3-117 Intelli-Plate 96-2 Low Profile 80 plate case
HR3-118 CrystalMation Intelli-Plate 96-3 low-profile 20 plate case
HR3-119 CrystalMation Intelli-Plate 96-3 low-profile 80 plate case
HR3-143 Intelli-Plate 96-2 LVR 10 plate case
HR3-145 Intelli-Plate 96-2 LVR 40 plate case
HR3-183 Intelli-Plate 96-3 LVR 10 plate case
HR3-185 Intelli-Plate 96-3 LVR 40 plate case
HR3-297 Intelli-Plate 96-2 Original 10 plate case
HR3-299 Intelli-Plate 96-2 Original 40 plate case
HR8-171 Intelli-Plate Flat Shelf 10 plate case
HR8-172 Intelli-Plate Flat Shelf 40 plate case
HR3-163 Intelli-Plate 96-2 Shallow Well 10 plate case
HR3-164 Intelli-Plate 96-2 Shallow Well 40 plate case
HR3-181 Intelli-Plate 96-2 Shallow Well Low Profile 20 plate case
HR3-182 Intelli-Plate 96-2 Shallow Well Low Profile 80 plate case

Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理(活性荧光染料)   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理(蛋白结晶) whatman代理(滤膜滤纸) GE代理(蛋白纯化) Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理(糖蛋白分析产品)  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

Hampton 银弹 Silver Bullets

Hampton 银弹 Silver Bullets

Applications

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Developed at Hampton Research
Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

Description
Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3

Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3

X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.

Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests

The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:

•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal

The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ºC, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.

Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.

Silver Bullets reagent volume is 0.5 ml (each well).

ThermoFluor is a registered trademark of Johnson & Johnson.

Hampton  Silver Bullets Bio

Silver Bullets • Silver Bullets Bio

Silver Bullets • Silver Bullets Bio

上海金畔生物作为Hampton蛋白结晶产品的代理商,竭诚为您服务,欢迎新老客户咨询。

Applications 应用

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

 

Developed at Hampton Research
Two sets of 96 reagents, composed of more than 1,090 chemicals, of which more than 400 are uniqu

Please note, the Silver Bullets Bio Screen is temporarily unavailable as a new formulation is in development

 

Hampton 蛋白质结晶试剂和耗材介绍

特色

Hampton 蛋白质结晶试剂和耗材介绍

美国Hampton Research公司位于加利福尼亚州,是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材,同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线,不仅为全世界晶体研究人员的工作带来了便捷,而且也受益于科研人员的回馈不断发展自己,目前已成为晶体研究领域最受信赖的品牌之一。
上海金畔生物作为Hampton research品牌蛋白结晶产品的代理商,我们将竭诚为您服务,欢迎新老客户咨询。

Hampton research主要产品:

产品分类 特点描述
Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液
Optimize Reagents 结晶级别盐类,聚合物,有机溶液或缓冲液
Crystallization Plates, Hardware & Accessories 结晶板(悬滴法和坐滴法,微量透析,微配液,自由界面扩散,凝胶以及无容器悬浮法)及其他配件
Cryocrystallography 低温晶体学耗材如Crystalcap system,cryoloops
Goniometer Heads&Supplies 测角器头和配件
Protein Crystallization Standards 结晶级别标准蛋白
Custom Shop

Crystallization Reagents

定制试剂盒和结晶溶液内的单一组分
StockOptions Kits 内含盐类,缓冲液和冷冻液的简便型试剂盒
Tools,Seeding&Resin 结晶用探针,结晶笔,seeding法结晶工具
Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂

美国Hampton Research公司是世界上著名的蛋白质晶体试剂供应商,该公司的产品目录是由上海金畔生物科技有限公司提供,该目录分为12个大类和几十个小类。

Hampton蛋白质结晶试剂及耗材分类

第一类:Crystallization Screens   用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液

1.PCT Pre-Crystallization Test(PCT预结晶试验)

应用:结晶筛选,确定相应的样品浓度,PCT试验用来预结晶结晶筛选,以确定适当的蛋白质浓度

  1. Slice pH(PH切片)

应用:溶解度和结晶筛选

  1. Solubility & Stability Screen(溶解度和稳定性的屏幕)

应用:溶解性和稳定性屏幕的设计,以协助识别解决方案的条件,促进蛋白质的溶解性和稳定性,并尽量减少蛋白质沉淀。

  1. Index

应用:不同的试剂系统的结晶屏幕的蛋白质复合物,多肽,核酸,水溶性小分子。

  1. Crystal Screen(晶体屏幕)

应用:

主屏幕的可溶性肽,蛋白质,核酸,和水溶性小分子。稀疏矩阵的加法屏幕

5.PEG/Ion Screen & PEG/Ion HT Screen 用于可溶性蛋白的结晶

6.PEG/Ion 2 Screen?用于可溶性生物大分子结晶

7.Crystal Screen? & Crystal Screen? HT 用于蛋白质,复合物,缩氨酸,核酸以及水溶性小分子的传统的经典结晶试剂

8.Crystal Screen 2?用于蛋白质,缩氨酸,核酸以及水溶性小分子的结晶

9.Crystal Screen Lite?用于蛋白质,缩氨酸和核酸的结晶试剂

10.Crystal Screen Cryo?用于蛋白质,缩氨酸,核酸的结晶

11.Grid ScreenTM用于蛋白质的结晶,分为七小类。

12.Quik Screen?用于可溶性蛋白的结晶

13.Quik Optimize?快速优化试剂,与Quik Screen? 或 custom screen配套使用

14.Low Ionic Strength Screen?用于完整的单克隆抗体,单克隆抗体片段以及低离子浓度很少溶解的蛋白的结晶

15.Nucleic Acid Mini Screen?用于核酸片段的结晶化

16.Natrix?用于核酸,蛋白核酸聚合物的结晶

17.MembFac?& MembFac? HT用于膜蛋白的结晶

18.Additive Screen?增强或改变样品可溶性的试剂

19.Detergent Screen?去垢剂

20.Heavy Atom Screens重原子结晶试剂

21.Izit Crystal Dye?蛋白染色试剂,用于区分蛋白晶体和盐晶体

第二类:Custom Shop Crystallization Reagents  结晶试剂盒中单独的试剂

  1. Solubility & Stability Individual Reagents
  2. Slice pH Individual Reagents
  3. Ionic Liquid Screen Individual Reagents
  4. Proti-Ace & Proti-Ace 2 Individual Reagents
  5. PCT Individual Reagents
  6. Silver Bullets & Silver Bullets HT Individual Reagents
  7. Silver Bullets Bio & Silver Bullets Bio HT Individual Reagents
  8. Index • Index HT Individual Reagents
  9. PEGRx 1 Individual Reagents
  10. PEGRx 2 Individual Reagents
  11. SaltRx Individual Reagents
  12. PEG/Ion Individual Reagents
  13. Crystal Screen Lite Individual Reagents
  14. Crystal Screen Cryo Individual Reagents
  15. Crystal Screen 2 Cryo Individual Reagents
  16. Grid Screen Ammonium Sulfate Individual Reagents
  17. Grid Screen PEG 6000 Individual Reagent
  18. Grid Screen Sodium Chloride Individual Reagents
  19. Grid Screen PEG/LiCl Individual Reagents
  20. Quik Screen Individual Reagents
  21. Natrix Individual Reagents

第三类:Optimize Reagents 优化试剂

1.Optimize-Polymers聚合体

2.Optimize-Organics (Volatile) 挥发性有机物

3.Optimize-Organics (Non-Volatile)非挥发性的有机物

4.Optimize-Salts 盐类

5.Optimize-Buffers缓冲液

6.Optimize-Solubilizing Agents (NDSB) 溶解试剂NDSB

7.Optimize-Reducing Agent还原剂-TCEP氢氯化物

8.Optimize-Cryoprotectants

9.Optimize-Oils用于结晶的油类

10.Optimize-Silica Hydrogel硅胶

11.Optimize-LM Agarose低熔点琼脂糖

第四类:StockOptions Kits

  1. StockOptions Salt
  2. StockOptions pH
  3. StockOptions Buffer
  4. CryoPro

第五类:Crystallization Plates, Hardware & Accessories 结晶耗材

1.24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种

2.48 Well Crystallization Plates 48孔晶体板

3.72 Well Crystallization Plates72孔晶体板

4.96 Well Crystallization Plates 96孔晶体板

5.384 Well Crystallization Plates 384孔晶体板-用于坐滴的结晶

6.Cover Slides & Related Tools

7.Glass Sitting Drop Rods用于坐滴的结晶

8.Micro-Bridges?微桥-坐滴结晶的支撑物

9.Glass Plates – 3 and 9 Well3孔和9孔玻璃板

10.Sealants, Sealing Grease, Oils, Applicator密封油脂,油及其它器具

11.Sealing Films, Tapes, Mats & Covers

12.Microdialysis Buttons, Rods & Membranes微量渗析结晶化

13.Granada Crystallization Box

第六类:Tools, Seeding & Resin(结晶工具、树脂)

  1. Tungsten-carbide Glass Cutter
  2. X-Acto Gripster Knife
  3. Micro-Tools™ Set
  4. Micro-Tools II™ Set
  5. Forceps
  6. Crystal Probe™
  7. Crystal Pencil
  8. Seed Bead
  9. Seeding Tool

第七类:Cryocrystallography

  1. CrystalCap HT™ Systems
  2. CrystalCap Magnetic™ Systems
  3. CrystalCap™ Systems
  4. CryoLoops & MicroTubes
  5. Cryo Tools
  6. Canes, Sleeves, Coders & Boxes
  7. Dewars

第八类:Capillary Mounts & Supplies(毛细管座椅支架供应)

第九类:Goniometer Heads & Supplies(测角头及用品)

第十类:Xenon Derivatization(氙气衍生物)

第十一类:Labels & Pens(标签和记录笔)

第十二类:Protein Crystallization Standards(蛋白质结晶的标准)

Hampton代理,Hampton上海代理,Hampton北京代理,Hampton广州代理,Hampton总代理,Hampton一级代理,Hampton特约代理,Hampton现货上海金畔生物科技有限公司Hampton专业代理。

Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理(活性荧光染料)   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理(蛋白结晶) whatman代理(滤膜滤纸) GE代理(蛋白纯化) Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理(糖蛋白分析产品)  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

Hampton GRAS 1 • GRAS 2 Screens

Hampton GRAS 1 • GRAS 2 Screens

stallization screen for proteins, including monoclonal antibodies, where Polyethylene glycol is the primary, and Salt the secondary reagent, sampling pH 4-9
GRAS 1 • GRAS 2 ScreensGRAS 1 • GRAS 2 Screens Identify GRAS based reagents that promote crystallization of biotherapeutics for bioprocess, bioformulation, and continuous flow manufacturing applications
Features

Developed at Hampton Research
Generally Recognized As Safe reagent formulation
Samples pH 4.0 – 9.0 without an added buffer
Primary crystallization reagent:

GRAS Screen 1: PEG 300, 400, MME 550, & 600
GRAS Screen 2: PEG 1,000, MME 2,000, 3,350 & 4,000
Secondary crystallization reagent:

24 unique salts
Compatible with Vapor diffusion, microbatch, free interface diffusion
Reagents soluble between 4°C and 30°C

Description
GRAS Screen™ 1 and GRAS Screen™ 2 were developed by Hampton Research for the crystallization of proteins, including monoclonal antibodies. Each of the chemicals in GRAS Screen 1 and 2 have been used under one or more of the following categories. As (1) a Generally Recognized As Safe (GRAS) substance, (2) a pharmaceutical excipient, (3) a normal physiological constituent, (4) a metabolic byproduct, and/or (5) a Everything Added to Food in the United States (EAFUS) substance.

GRAS Screen 1 samples four low molecular weight Polyethylene glycols (300, 400, MME 550, and 600) versus twenty-four salts, encompassing pH 4-9. GRAS Screen 1 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 1 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

GRAS Screen 2 samples four medium molecular weight Polyethylene glycols (1,000, MME 2,000, 3,350, & 4,000) versus twenty-four salts, encompassing pH 4-9. GRAS Screen 2 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 2 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

CAT NO NAME DESCRIPTION
HR2-451 GRAS Screen 1 1 ml, Deep Well block format
Price Quantity
$245.00
CAT NO NAME DESCRIPTION
HR2-452 GRAS Screen 2 1 ml, Deep Well block format
Price Quantity
$245.00

Hampton Solubility & Stability Screen

Hampton Solubility & Stability Screen

Solubility & Stability Screen(溶解度和稳定性的屏幕)

应用:溶解性和稳定性屏幕的设计,以协助识别解决方案的条件,促进蛋白质的溶解性和稳定性,并尽量减少蛋白质沉淀。

Solubility & Stability Screen

Solubility & Stability Screen

Solubility & Stability Screen

Solubility & Stability Screen HR2-072

Applications

A solubility screen, a stability screen, an additive screen for protein assays including ThermoFluor and crystallization
Preformulation screening

 

Compatible with Thermofluor , Differential Scanning Fluorimetry (DSF), and Protein Thermal Shift assays
96 sterile filtered reagents
17 classes of reagents and excipients
Highly concentrated (10x) reagent formulation
Developed at Hampton Research

Features

Methodologies

Thermal shift assay
Dynamic Light Scattering
Use Solubility & Stability Screen along side Slice pH and Solubility & Stability Screen 2 to optimize sample buffer
Description

Solubility & Stability Screen is designed to assist in the identification of solution conditions which promote protein solubility and stability, and minimize protein precipitation. Solubility & Stability Screen is a solubility screen, a stability screen, and may also be used as an additive screen in the presence of a crystallization reagent.

The Hampton Research Solubility and Stability Screen can evaluate protein solubility, stability and crystallization in the presence of 94 different chemical additives sampling 17 different classes of reagents plus two controls.

Protein solubility and stability are universally required in a wide range of applications, including general biochemical studies, the preparation of proteins in pharmaceuticals, structural biology and crystallization.1-11The preparation of a concentrated, soluble and stable protein sample can often be a difficult task as proteins often aggregate, precipitate or denature.

Thermofluor (also known as Differential Scanning Fluorimetry, DSF and Protein Thermal Shift) provides a rapid, convenient and effective means of identifying subsaturating solution conditions that may also correlate with protein heterogeneity and poor crystallization outcomes.12

Protein solubility and stability is affected by many different chemical factors including pH, buffer type, chemical additives and excipients. pH and buffer type are dominant protein solubility and stability variables and can be evaluated and optimized using the Hampton Research Slice pH kit HR2-070. Slice pH evaluates protein solubility, stability and crystallization versus 20 different buffers over the pH range 3.5-9.6. Chemical additives influencing protein solubility and stability can be evaluated using the Solubility & Stability Screen.

It is widely accepted that protein solubility and stability can be increased by the use of chemical additives.5,15Seventeen classes of reagents are sampled by the Solubility & Stability Screen and each of these classes has been reported as important in improving sample solubility and stability.2-11

The Solubility & Stability Screen is a set of 94 high purity reagents formulated in high purity water (NCCLS/ASTM Type 1+) at 25°C and are 0.22 micron sterile filtered. The 94 solubility and stability reagents are formulated at 2 to 10 times their recommended working concentration. The remaining two reagents are water and a negative (TCA) control. A water control demonstrates the effect of diluting sample as well as sample buffer concentration. TCA, the negative control, demonstrates total sample precipitation, loss of sample solubility and loss of sample stability. The effects of the Solubility & Stability reagents can be compared with this negative control to assist in the identification of reagents promoting sample solubility and stability. 500 microliters of each reagent is supplied in a sterile 96 well polypropylene Deep Well block. The Solubility & Stability Screen reagents are compatible with the sitting or hanging drop vapor diffusion, microbatch, free interface diffusion, sandwich drop vapor diffusion, and dialysis crystallization methods utilizing water soluble reagents.

Hampton 24孔坐滴板 Cryschem Plate

Hampton 24孔坐滴板 Cryschem Plate

Applications

 Sitting drop crystallization

  Heavy atom soaks

  Seeding

Features

Optically clear, concave sitting drop post
Cryschem Plate – Larger footprint and round reagent reservoir
Cryschem M – Microplate footprint with round reagent reservoir
Cryschem S – Microplate footprint with square reagent reservoir

Compatible with Formulatrix Formulator

Smooth, flat top for easy seal
Each plate supplied with a cover

Materials

  Optically clear polystyrene

 Description

The Cryschem Plate is a 24 well sitting drop plate that is sealed with clear sealing tape. The polystyrene plate is supplied with a raised cover so the plate can also be sealed with vacuum grease and plain glass or plastic cover slides (22 mm diameter). The stackable, 24 well plates have distortion-free, flat bottom wells and a concave sitting drop post in the center of the well for concentric equilibration of the drop with the reagent. Each plate is individually sealed. The top of the plate and each of the 24 wells is a single, flat plane for smooth and easy seal application. Well spacing provides for a large seal area between wells to prevent cross contamination and evaporation.

上海金畔生物作为Hampton蛋白结晶产品的代理商,竭诚为您服务,欢迎新老客户咨询。

Cryschem Plate is large plate footprint with circular reagent reservoir. Cryschem plate approximate size: 15.1 cm x 10.6 cm x 2.2 cm. Approximate well size: 1.6 cm x 1.5 cm. Typical fill volume: 500 to 1,000 µl. Well capacity: 1.5 ml. Maximum drop volume on post: 40 µl. Recommended seal: HR3-511 Crystal Clear Sealing Tape (1.88 inch x 43.7 yard roll, with cutter) or HR4-511 Crystal Clear Sealing Tape (1.88 inch x 60 yard roll, without cutter).

Cryschem M Plate is microplate footprint with circular reagent reservoir. Cryschem M microplate approximate size: 128 mm x 86 mm x 14 mm. Approximate circular reservoir size: 15 mm wide x 9 mm deep. Typical reservoir volume: 500 µl. Well capacity: 1.1 ml. Maximum drop volume on post: 30 µl. Recommended seal: HR3-609 Crystal Clear Sealing Film (100 pack), HR4-521 ClearSeal Film (100 pack) or HR4-506 Crystal Clear Sealing Tape (3 inch x 55 yard roll, without cutter). HR4-508 Crystal Clear Sealing Tape (0.75 inch x 650 inch, with cutter) seals a single row at a time.

Cryschem S Plate is microplate footprint with square reagent reservoir. Cryschem S microplate approximate size: 128 mm x 86 mm x 14 mm. Approximate square reservoir size: 15 mm wide x 9 mm deep. Typical reservoir volume: 500 µl. Well capacity: 1.1 ml. Maximum drop volume on post: 30 µl. Recommended seal: HR3-609 Crystal Clear Sealing Film (100 pack), HR4-521 ClearSeal Film (100 pack) or HR4-506 Crystal Clear Sealing Tape (3 inch x 55 yard roll, without cutter). HR4-508 Crystal Clear Sealing Tape (0.75 inch x 650 inch, with cutter) seals a single row at a time.

Cryschem Plate

Cryschem plate

CAT NO NAME DESCRIPTION
HR3-158 Cryschem Plate 24 plate case
HR3-160 Cryschem Plate 100 plate case
HR1-002 Cryschem M Plate (round reagent reservoir) 50 plate case
HR3-308 Cryschem S Plate (square reagent reservoir) 50 plate case

Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理(活性荧光染料)   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理(蛋白结晶) whatman代理(滤膜滤纸) GE代理(蛋白纯化) Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理(糖蛋白分析产品)  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

Hampton晶体删选试剂盒

Hampton晶体删选试剂

1.Screens 晶体删选试剂盒

产品名称 货号 用途备注
Index Kit HR2-144 用于生物大分子结晶
CrystalScreen Kit HR2-110 用于蛋白质,核酸水溶性小分子结晶
Crystal Screen 2 Kit HR2-112
Pegrx 1 HR2-082 用于生物大分子结晶
Pegrx 2 HR2-084 用于生物大分子结晶
Peg/Ion Screen Kit HR2-126 用于生物大分子结晶
Peg/Ion 2 Screen™ HR2-098 用于生物大分子结晶
Saltrx Kit HR2-108 用于生物大分子结晶
Membfac Kit HR2-114 用于膜蛋白和溶解度有限的样品结晶
Crystal Screen Lite HR2-128 用于蛋白质,缩氨酸,核酸的结晶
Natrix Kit HR2-116 用于核酸和蛋白核酸复合物的结晶
Crystal Screen Cyro Kit HR2-122 用于蛋白质,可溶性缩氨酸,核酸和水溶性小分子的初次低温结晶
Nucleic Acid Mini Screen Kit HR2-118 用于核酸片段的结晶
Low Ionic Strength Screen HR2-120 用于完整单抗,单抗片段以及在低离子浓度下溶解度较低的样品结晶
Silver Bullet & Silver Bullet Bio HR2-078 用于生物大分子的初次和二次结晶
HR2-080
Peg/Tacsimate Ph5.8 Crystallization Reagent HR2-090
Peg/Tacsimate Ph 6.8 Crystallization Reagent HR2-092
Peg/Tacsimate Ph 7.8 Crystallization Reagent HR2-094
Additive Screen™ HR2-428 利用样品间和样品溶剂间相互作用来优化晶体生长和改变样品溶解度
产品名称 货号 用途备注
Detergent Screen 1 HR2-410 改善疏水作用引起的非特异性聚集
Detergent Screen 2 HR2-411
Detergent Screen 3 HR2-412
Detergent Screen HR2-408
Heavy Atom Screen Pt HR2-442 Heavy Atoms For Multiple Isomorphous Replacement
Heavy Atom Screen Au HR2-444
Heavy Atom Screen Hg HR2-446
Heavy Atom Screen M1 HR2-448
Heavy Atom Screen M2 HR2-450
I3c Phasing Kit HR3-133

2.Custom Shop Crystallization Reagents 结晶试剂盒中单独的试剂

  1. Optimize™ Reagents晶体生长条件优化试剂

⑴ Optimize™ – Polymers聚合体

⑵ Optimize™- Organics (Volatile) 挥发性有机物

⑶ Optimize™- Organics (Non-Volatile)非挥发性的有机物

⑷ Optimize™- Salts 盐类

⑸ Optimize™- Buffers缓冲液

⑹ Optimize™- Solubilizing Agents (NDSB) 溶解试剂NDSB

⑺ Optimize™ – Reducing Agent还原剂-TCEP氢氯化物

⑻ Optimize™ – Cryoprotectants

⑼ Optimize™ – Oils用于结晶的油类

⑽ Optimize™ – Silica Hydrogel硅胶

⑾ Optimize™ – LM Agarose低熔点琼脂糖
5.Crystallization Plates, Hardware & Accessories结晶耗材
⑴ 24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种
⑵ 48 Well Crystallization Plates 48孔晶体板
⑶ 72 Well Crystallization Plates72孔晶体板
⑷ 96 Well Crystallization Plates 96孔晶体板等