Hampton 银弹 Silver Bullets

Hampton 银弹 Silver Bullets

Applications

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Developed at Hampton Research
Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

Description
Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3

Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3

X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.

Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests

The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:

•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal

The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ºC, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.

Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.

Silver Bullets reagent volume is 0.5 ml (each well).

ThermoFluor is a registered trademark of Johnson & Johnson.

Hampton  Silver Bullets Bio

Silver Bullets • Silver Bullets Bio

Silver Bullets • Silver Bullets Bio

上海金畔生物作为Hampton蛋白结晶产品的代理商,竭诚为您服务,欢迎新老客户咨询。

Applications 应用

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

 

Developed at Hampton Research
Two sets of 96 reagents, composed of more than 1,090 chemicals, of which more than 400 are uniqu

Please note, the Silver Bullets Bio Screen is temporarily unavailable as a new formulation is in development

 

Hampton50号毛细管Glass Number 50 Capillaries

Hampton50号毛细管Glass Number 50 Capillaries

Glass Number 50 Capillaries

应用:

  X-ray data collection

  Liquid-liquid diffusion crystallization

  Gel acupuncture crystallization

Features

  Glass Number 50 (borosilicate)

  Thin walled – 10 micron

      上海金畔生物作为Hampton蛋白结晶产品的代理商,竭诚为您服务,欢迎新老客户咨询。

Description 描述
Glass Number 50 (borosilicate glass) capillaries that are extremely thin walled (approximately 10 micron wall thickness). The length of the capillary has a well defined diameter, with one end having a funnel shape and the other end closed. Glass capillaries have a wall thickness of 0.01 mm and an overall length of 80 mm +/- 5 mm. Glass capillaries are available in a wide range of outside diameters from 0.1 mm to 2.0 mm. They are designed to mount, hold, and store small molecule and biological macromolecular crystals for x-ray data collection. Capillaries can also be used for crystal density measurements and crystal growth experiments. The capillaries can be sealed tightly against moisture and gases using wax, epoxy, or other sealing materials.

In determining what glass or quartz capillary is right for you, please refer to the “Linear Absorption Coefficient µ cm-1” table. This table indicates the amount of radiation that is absorbed by the capillary during x-ray data collection.

For 0.1 mm to 2.0 mm capillaries the open end capillary tube base size is 3.0 +/- 0.15 mm OD x 0.13 +/- 0.10 mm Wall thickness.

The Diameter is measured 40 mm from the closed end. The tolerances are as follows

Glass Number 50 Capillaries

Glass Number 50 Capillaries

 

0.1 mm -0.075 / +0.075 mm
0.2 mm -0.050 / +0.050 mm
0.3 mm -0.050 / +0.100 mm
0.4 mm -0.100 / +0.100 mm
0.5 mm -0.100 / +0.100 mm
0.6 mm -0.100 / +0.100 mm
0.7 mm -0.150 / +0.150 mm
0.8 mm -0.150 / +0.150 mm
0.9 mm -0.150 / +0.150 mm
1.0 mm -0.150 / +0.250 mm
1.5 mm -0.250 / +0.250 mm
2.0 mm -0.250 / +0.250 mm

Capillaries have only been tested at atmospheric pressure (760 mmHg (torr), 29.92 inHg, 14.696 psi). Use at other pressures has not been tested.

Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理(活性荧光染料)   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理(蛋白结晶) whatman代理(滤膜滤纸) GE代理(蛋白纯化) Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理(糖蛋白分析产品)  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

Hampton蛋白结晶产品目录

Hampton蛋白结晶产品目录

产品编号 中文品名 英文品名
HR4-126 11 刀片替换装 Replacement #11 Blade
HR4-904 CrystalCap Magnetic Vial CrystalCap™, Vial only
HR4-729 CrystalWand Magnetic, Straight CrystalWand™ Magnetic, Straigh
HR4-837 Micro-Tools II Set Micro-Tools II™ Set
HR4-811 Micro-Tools Set Micro-Tools™ Set
HR3-172 VDX 24孔板,带密封剂 VDX™ Plate, with sealant
HR4-902 带磁力底座 CrystalCap™, without Vial
HR4-733 带磁力底座 CrystalCap™, with Vial
HR4-745 带铜管磁力底座 CrystalCap™ Copper 18 mm, with
HR4-708 冻存套 CryoSleeve™
HR3-217 硅化方形盖玻片22mmx0.22mm 22 mm x 0.22 mm Siliconized Sq
HR4-671 夹钳-弯 Vial Clamp™ – Curved, 45°/135°
HR4-670 夹钳-直 Vial Clamp™ – Straight
HR4-316 Super Glue
HR4-506 胶带 3 inch wide x 55 yard Crystal
HR3-083 结晶板 2孔 MRC 2 Well Crystallization Pla
HR3-125 结晶板 3孔 3 Well Crystallization Plate (
HR4-217 晶体探针 Crystal Probe
HR4-662 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR4-673 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR4-674 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR4-675 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR1-008 液氮罐 CX100 (含运输箱) CX100 Cryo-Express Dry Shipper
HR2-912-37   Crystal Screen 2™ #37
HR2-501   50% v/v Jeffamine® M-600® pH 7
HR2-509   1.0 M BICINE
HR2-517   100% Polyethylene glycol 300
HR2-523   50% w/v Polyethylene glycol 1,
HR2-525   50% w/v Polyethylene glycol 1,
HR2-529   50% w/v Polyethylene glycol 4,
HR2-533   50% w/v Polyethylene glycol 6,
HR2-537   1.0 M Magnesium formate dihydr
HR2-541   3.5 M Ammonium sulfate
HR2-545   2.0 M Lithium sulfate monohydr
HR2-547   7.0 M Sodium formate
HR2-549   1.6 M Sodium citrate tribasic
HR2-551   4.0 M Sodium phosphate monobas
HR2-557   2.0 M Calcium chloride dihydra
HR2-563   1.0 M Zinc acetate dihydrate
HR2-102   StockOptions™ Hepes
HR2-103   StockOptions™ Bis-Tris Propane
HR2-104   StockOptions™ Citric Acid
HR2-106   StockOptions™ Bis-Tris
HR2-107   SaltRx™ 1
HR2-109   SaltRx™ 2
HR2-110   Crystal Screen™
HR2-112   Crystal Screen 2™
HR2-114   MembFac™
HR2-116   Natrix™
HR2-117   Natrix™ 2
HR2-118   Nucleic Acid Mini Screen™
HR2-126   PEG/Ion Screen™
HR2-235   StockOptions™ Sodium Citrate
HR2-237   StockOptions™ Tris Hydrochlori
HR2-239   StockOptions™ Sodium Cacodylat
HR2-241   StockOptions™ pH
HR2-245   StockOptions™ Salt
HR2-249   StockOptions™ Succinic Acid
HR2-320   Seed Bead™
HR2-406   Detergent Screen HT™
HR2-428   Additive Screen™
HR2-434   Reductive Alylation Kit
HR2-442   Heavy Atom Screen™ Pt
HR2-444   Heavy Atom Screen™ Au
HR2-446   Heavy Atom Screen™ Hg
HR2-448   Heavy Atom Screen M1™
HR2-450   Heavy Atom Screen M2™
HR2-082   PEGRx™ 1
HR2-084   PEGRx™ 2
HR2-096   Silver Bullets™
HR2-098   PEG/Ion 2 Screen™
HR4-315   CrystalWand™ Magnetic, 45° McM
HR4-350   Large Applicator (200 & 350 µl
HR4-625   Mounted CryoLoop™ – 20 micron,
HR4-637   CryoTong™ 18 mm (standard 110
HR4-709   CryoCane™ 5 Vial Holder
HR4-711   CryoCane™ 6 Vial Holder
HR2-211   Grid Screen™ Ammonium Sulfate
HR2-213   Grid Screen™ PEG 6000
HR2-227   StockOptions™ Polymer
HR2-233   StockOptions™ Sodium Acetate
HR4-931   CryoLoop™ – 20 micron, 0.2 – 0
HR4-945   Mounted CryoLoop™ – 20 micron,
HR4-947   Mounted CryoLoop™ – 20 micron,
HR4-953   Mounted CryoLoop™ Sampler – 20
HR4-955   Mounted CryoLoop™ – 20 micron,
HR4-957   Mounted CryoLoop™ – 20 micron,
HR4-959   Mounted CryoLoop™ – 20 micron,
HR4-961   Mounted CryoLoop™ – 20 micron,
HR4-963   Mounted CryoLoop™ – 20 micron,
HR4-970   Mounted CryoLoop™ – 20 micron,
HR4-971   Mounted CryoLoop™ – 20 micron,
HR4-989   CryoLoop™ – 10 micron, 0.3 – 0
HR2-128   Crystal Screen Lite™
HR2-138   Additive Screen HT™
HR2-140   PCT™ – Pre-Crystallization T
HR4-313   Mounted CryoLoop™ – 20 micron,
HR2-601   100% Polyethylene glycol 200
HR2-607   50% w/v Polyethylene glycol 10
HR2-609   30% w/v Polyethylene glycol 20
HR2-611   100% Polyethylene glycol monom
HR2-613   50% w/v Polyethylene glycol mo
HR2-615   50% w/v Polyethylene glycol mo
HR2-625   6.0 M 1,6-Hexanediol
HR2-637   5.0 M Sodium chloride
HR2-723   1.0 M BICINE pH 9.0
HR2-755   100% Tacsimate™ pH 7.0
HR2-761   3.0 M DL-Malic acid pH 7.0
HR2-801   TCEP hydrochloride
HR2-821   2.0 M Magnesium sulfate heptah
HR2-829   100% Tacsimate™ pH 8.0
HR2-857   4.0 M TMAO
HR2-859   100% Polyethylene glycol 600
HR2-910-08   Crystal Screen™ #08
HR2-144   Index™
HR4-993   Mounted CryoLoop™ Sampler – 10
HR4-995   Mounted CryoLoop™ – 10 micron,
HR4-999   Mounted CryoLoop™ – 10 micron,
HR6-146   1.0 mm Quartz Capillary
HR8-034   Cryo Puck Carrier
HR8-035   Porcupine Push Tool
HR8-036   Puck Wand
HR8-037   Cryo Puck Base Removal Platfor
HR8-038   Angled Cryo Puck Tong
HR8-039   Cryo Puck Dewar Loading Tool
HR8-114   CrystalCap HT™, Vial only
HR8-133   Seeding Tool
HR8-173   CrystalCap™ Copper HT, 0.025 -
HR8-174   CrystalCap™ Copper HT, 0.05 -
HR8-175   CrystalCap™ Copper HT, 0.1 – 0
HR8-176   CrystalCap™ Copper HT, 0.2 – 0
HR2-575   1.0 M Sodium cacodylate trihyd
HR2-581   1.0 M Hydrochloric acid

低温泡沫杜瓦瓶 Spearlab Cryogenic Foam Dewars

低温泡沫杜瓦瓶 Spearlab Cryogenic Foam Dewars
Spearlab Cryogenic Foam Dewars
Spearlab Dewars
Spearlab Cryogenic Foam Dewars
HR4-662 Cryogenic Foam Dewar (500 ml) – Small  500ml规格
Spearlab Cryogenic Foam Dewars
HR4-675 – Spearlab Cryogenic Foam Dewar (2 liter)  2L规格
低温泡沫杜瓦瓶 Spearlab Cryogenic Foam Dewars

Applications 应用

    Cryocrystallography 蛋白结晶学

Features

  Each dewar is supplied with a lid  每个杜瓦瓶都配有盖子

  Proprietary foam construction (USPTO # 7,971,744)

  Mini (130 ml), Small (500 ml), Standard (800 ml), Large (1400 ml) and Tall (1800 ml)

   Reduced ice formation on dewar lip

  Easier to dry

   Foam construction reduces slipping

Description
Spearlab FD-130 Cryogenic Foam Dewar (130 ml) – Mini : Small, hexagonal outside, hand held foam dewar, maximum volume 175 ml. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 2 inches in diameter by 3.5 inches deep, so that it easily holds 130 ml and a maximum volume of 175 ml of liquid nitrogen. The HR4-676 has an overall height of approximately 4.5 inches without cover and the width is 3.5 inches at the top and 3.75 inches on the bottom (measured on the flats of the hexagon). Circular cover is approximately 0.5 inches tall x 3.5 inches diameter.

Spearlab Cryogenic Foam Dewar (500 ml) – Small: The small foam dewar shape is circular, with a protruding handle, so that it resembles a large teardrop. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 4.5 inches in diameter by 2.8 inches deep, so that it easily holds 500 ml of liquid nitrogen.

Spearlab FD-800 Cryogenic Foam Dewar (800 ml) – Standard: The standard foam dewar shape is circular, with a protruding handle, so that it resembles a large teardrop. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 5.8 inches in diameter by 2.8 inches deep, so that it easily holds 800 ml of liquid nitrogen.

Spearlab FD-1400 Cryogenic Foam Dewar (1400 ml) – Large: The large foam dewar shape is circular, with a protruding handle, so that it resembles a large teardrop. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 6.3 inches in diameter by 3.8 inches deep, so that it easily holds 1400 ml of liquid nitrogen. Overall height is approximately 6 inches without cover and the width is 9 inches on the short axis and 11 inches on the long axis. Cover approximately 0.5 inches tall x 9 inches diameter.

Spearlab FD-1800 Cryogenic Foam Dewar (1800 ml) – Tall: The tall foam dewar shape is a tapered octagon on the outside with a cylindrical interior. The tapered octagon features a wide, stable base. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 3.5 inches in diameter by 12.5 inches deep, so that it holds approximately 1,800 ml of liquid nitrogen. The outside dimensions of the tall dewar are 15.25 inches tall with a 6.25 inches wide top and a 7.75 inches wide base (measured on the flats of the octagon). The cover is 6.5 inches in diameter and 0.5 inches thick.

The patented (USPTO # 7,971,744) design of the Spearlab Cryogenic Foam Dewar makes it easy to handle and safer to use than a traditional low profile glass dewar. Also, because of its lower thermal mass, a foam vessel will cause less boil off when it is filled with liquid nitrogen. Additionally, the dewar will accumulate less frost during regular use. The end result is that less liquid nitrogen is consumed.

The dewars are made from cross-linked polyethylene foam. A density of 4 pounds per cubic foot is used for the 130 ml and 800 ml dewars. A density of 2 pounds per foot is used for the 1400 ml and 2 liter dewars. The higher density foam has significantly higher mechanical strength and stiffness. The lower density foam has slightly lower thermal conductivity. Like most lightweight insulation materials, the thermal conductivity of the foam is predominantly determined by the thermal conductivity of air, which eventually permeates the pores of the foam material.

CAT NO NAME DESCRIPTION
HR4-676 Cryogenic Foam Dewar (130 ml) – Mini each
HR4-662 Cryogenic Foam Dewar (500 ml) – Small each
HR4-673 Cryogenic Foam Dewar (800 ml) – Standard each
HR4-674 Cryogenic Foam Dewar (1400 ml) – Large each
HR4-675 Cryogenic Foam Dewar (2 liter) – Tall each

Hampton GRAS 1 • GRAS 2 Screens

Hampton GRAS 1 • GRAS 2 Screens

stallization screen for proteins, including monoclonal antibodies, where Polyethylene glycol is the primary, and Salt the secondary reagent, sampling pH 4-9
GRAS 1 • GRAS 2 ScreensGRAS 1 • GRAS 2 Screens Identify GRAS based reagents that promote crystallization of biotherapeutics for bioprocess, bioformulation, and continuous flow manufacturing applications
Features

Developed at Hampton Research
Generally Recognized As Safe reagent formulation
Samples pH 4.0 – 9.0 without an added buffer
Primary crystallization reagent:

GRAS Screen 1: PEG 300, 400, MME 550, & 600
GRAS Screen 2: PEG 1,000, MME 2,000, 3,350 & 4,000
Secondary crystallization reagent:

24 unique salts
Compatible with Vapor diffusion, microbatch, free interface diffusion
Reagents soluble between 4°C and 30°C

Description
GRAS Screen™ 1 and GRAS Screen™ 2 were developed by Hampton Research for the crystallization of proteins, including monoclonal antibodies. Each of the chemicals in GRAS Screen 1 and 2 have been used under one or more of the following categories. As (1) a Generally Recognized As Safe (GRAS) substance, (2) a pharmaceutical excipient, (3) a normal physiological constituent, (4) a metabolic byproduct, and/or (5) a Everything Added to Food in the United States (EAFUS) substance.

GRAS Screen 1 samples four low molecular weight Polyethylene glycols (300, 400, MME 550, and 600) versus twenty-four salts, encompassing pH 4-9. GRAS Screen 1 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 1 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

GRAS Screen 2 samples four medium molecular weight Polyethylene glycols (1,000, MME 2,000, 3,350, & 4,000) versus twenty-four salts, encompassing pH 4-9. GRAS Screen 2 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 2 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

CAT NO NAME DESCRIPTION
HR2-451 GRAS Screen 1 1 ml, Deep Well block format
Price Quantity
$245.00
CAT NO NAME DESCRIPTION
HR2-452 GRAS Screen 2 1 ml, Deep Well block format
Price Quantity
$245.00

Hampton 蛋白质结晶试剂和耗材介绍

特色

Hampton 蛋白质结晶试剂和耗材介绍

美国Hampton Research公司位于加利福尼亚州,是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材,同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线,不仅为全世界晶体研究人员的工作带来了便捷,而且也受益于科研人员的回馈不断发展自己,目前已成为晶体研究领域最受信赖的品牌之一。
上海金畔生物作为Hampton research品牌蛋白结晶产品的代理商,我们将竭诚为您服务,欢迎新老客户咨询。

Hampton research主要产品:

产品分类 特点描述
Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液
Optimize Reagents 结晶级别盐类,聚合物,有机溶液或缓冲液
Crystallization Plates, Hardware & Accessories 结晶板(悬滴法和坐滴法,微量透析,微配液,自由界面扩散,凝胶以及无容器悬浮法)及其他配件
Cryocrystallography 低温晶体学耗材如Crystalcap system,cryoloops
Goniometer Heads&Supplies 测角器头和配件
Protein Crystallization Standards 结晶级别标准蛋白
Custom Shop

Crystallization Reagents

定制试剂盒和结晶溶液内的单一组分
StockOptions Kits 内含盐类,缓冲液和冷冻液的简便型试剂盒
Tools,Seeding&Resin 结晶用探针,结晶笔,seeding法结晶工具
Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂

美国Hampton Research公司是世界上著名的蛋白质晶体试剂供应商,该公司的产品目录是由上海金畔生物科技有限公司提供,该目录分为12个大类和几十个小类。

Hampton蛋白质结晶试剂及耗材分类

第一类:Crystallization Screens   用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液

1.PCT Pre-Crystallization Test(PCT预结晶试验)

应用:结晶筛选,确定相应的样品浓度,PCT试验用来预结晶结晶筛选,以确定适当的蛋白质浓度

  1. Slice pH(PH切片)

应用:溶解度和结晶筛选

  1. Solubility & Stability Screen(溶解度和稳定性的屏幕)

应用:溶解性和稳定性屏幕的设计,以协助识别解决方案的条件,促进蛋白质的溶解性和稳定性,并尽量减少蛋白质沉淀。

  1. Index

应用:不同的试剂系统的结晶屏幕的蛋白质复合物,多肽,核酸,水溶性小分子。

  1. Crystal Screen(晶体屏幕)

应用:

主屏幕的可溶性肽,蛋白质,核酸,和水溶性小分子。稀疏矩阵的加法屏幕

5.PEG/Ion Screen & PEG/Ion HT Screen 用于可溶性蛋白的结晶

6.PEG/Ion 2 Screen?用于可溶性生物大分子结晶

7.Crystal Screen? & Crystal Screen? HT 用于蛋白质,复合物,缩氨酸,核酸以及水溶性小分子的传统的经典结晶试剂

8.Crystal Screen 2?用于蛋白质,缩氨酸,核酸以及水溶性小分子的结晶

9.Crystal Screen Lite?用于蛋白质,缩氨酸和核酸的结晶试剂

10.Crystal Screen Cryo?用于蛋白质,缩氨酸,核酸的结晶

11.Grid ScreenTM用于蛋白质的结晶,分为七小类。

12.Quik Screen?用于可溶性蛋白的结晶

13.Quik Optimize?快速优化试剂,与Quik Screen? 或 custom screen配套使用

14.Low Ionic Strength Screen?用于完整的单克隆抗体,单克隆抗体片段以及低离子浓度很少溶解的蛋白的结晶

15.Nucleic Acid Mini Screen?用于核酸片段的结晶化

16.Natrix?用于核酸,蛋白核酸聚合物的结晶

17.MembFac?& MembFac? HT用于膜蛋白的结晶

18.Additive Screen?增强或改变样品可溶性的试剂

19.Detergent Screen?去垢剂

20.Heavy Atom Screens重原子结晶试剂

21.Izit Crystal Dye?蛋白染色试剂,用于区分蛋白晶体和盐晶体

第二类:Custom Shop Crystallization Reagents  结晶试剂盒中单独的试剂

  1. Solubility & Stability Individual Reagents
  2. Slice pH Individual Reagents
  3. Ionic Liquid Screen Individual Reagents
  4. Proti-Ace & Proti-Ace 2 Individual Reagents
  5. PCT Individual Reagents
  6. Silver Bullets & Silver Bullets HT Individual Reagents
  7. Silver Bullets Bio & Silver Bullets Bio HT Individual Reagents
  8. Index • Index HT Individual Reagents
  9. PEGRx 1 Individual Reagents
  10. PEGRx 2 Individual Reagents
  11. SaltRx Individual Reagents
  12. PEG/Ion Individual Reagents
  13. Crystal Screen Lite Individual Reagents
  14. Crystal Screen Cryo Individual Reagents
  15. Crystal Screen 2 Cryo Individual Reagents
  16. Grid Screen Ammonium Sulfate Individual Reagents
  17. Grid Screen PEG 6000 Individual Reagent
  18. Grid Screen Sodium Chloride Individual Reagents
  19. Grid Screen PEG/LiCl Individual Reagents
  20. Quik Screen Individual Reagents
  21. Natrix Individual Reagents

第三类:Optimize Reagents 优化试剂

1.Optimize-Polymers聚合体

2.Optimize-Organics (Volatile) 挥发性有机物

3.Optimize-Organics (Non-Volatile)非挥发性的有机物

4.Optimize-Salts 盐类

5.Optimize-Buffers缓冲液

6.Optimize-Solubilizing Agents (NDSB) 溶解试剂NDSB

7.Optimize-Reducing Agent还原剂-TCEP氢氯化物

8.Optimize-Cryoprotectants

9.Optimize-Oils用于结晶的油类

10.Optimize-Silica Hydrogel硅胶

11.Optimize-LM Agarose低熔点琼脂糖

第四类:StockOptions Kits

  1. StockOptions Salt
  2. StockOptions pH
  3. StockOptions Buffer
  4. CryoPro

第五类:Crystallization Plates, Hardware & Accessories 结晶耗材

1.24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种

2.48 Well Crystallization Plates 48孔晶体板

3.72 Well Crystallization Plates72孔晶体板

4.96 Well Crystallization Plates 96孔晶体板

5.384 Well Crystallization Plates 384孔晶体板-用于坐滴的结晶

6.Cover Slides & Related Tools

7.Glass Sitting Drop Rods用于坐滴的结晶

8.Micro-Bridges?微桥-坐滴结晶的支撑物

9.Glass Plates – 3 and 9 Well3孔和9孔玻璃板

10.Sealants, Sealing Grease, Oils, Applicator密封油脂,油及其它器具

11.Sealing Films, Tapes, Mats & Covers

12.Microdialysis Buttons, Rods & Membranes微量渗析结晶化

13.Granada Crystallization Box

第六类:Tools, Seeding & Resin(结晶工具、树脂)

  1. Tungsten-carbide Glass Cutter
  2. X-Acto Gripster Knife
  3. Micro-Tools™ Set
  4. Micro-Tools II™ Set
  5. Forceps
  6. Crystal Probe™
  7. Crystal Pencil
  8. Seed Bead
  9. Seeding Tool

第七类:Cryocrystallography

  1. CrystalCap HT™ Systems
  2. CrystalCap Magnetic™ Systems
  3. CrystalCap™ Systems
  4. CryoLoops & MicroTubes
  5. Cryo Tools
  6. Canes, Sleeves, Coders & Boxes
  7. Dewars

第八类:Capillary Mounts & Supplies(毛细管座椅支架供应)

第九类:Goniometer Heads & Supplies(测角头及用品)

第十类:Xenon Derivatization(氙气衍生物)

第十一类:Labels & Pens(标签和记录笔)

第十二类:Protein Crystallization Standards(蛋白质结晶的标准)

Hampton代理,Hampton上海代理,Hampton北京代理,Hampton广州代理,Hampton总代理,Hampton一级代理,Hampton特约代理,Hampton现货上海金畔生物科技有限公司Hampton专业代理。

Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理(活性荧光染料)   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理(蛋白结晶) whatman代理(滤膜滤纸) GE代理(蛋白纯化) Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理(糖蛋白分析产品)  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

Hampton 水晶透明密封带Crystal Clear Sealing Tape

Hampton 水晶透明密封带Crystal Clear Sealing Tape

Crystal Clear Sealing Tape

3 inch, 1.88 inch, 1.88 inch with dispenser, 0.75 inch with dispenser (left to right)

Crystal Clear Sealing Tape

0.75 inch Crystal Clear Sealing Tape

Applications  应用
Sealing tape used to seal sitting drop crystallization experiments

Description

These optically clear tapes are compatible with protein crystallization reagents. The polypropylene Crystal Clear Sealing Tapes use acrylic solvent based adhesives which are compatible with aqueous crystallization reagents.

Catalog number HR3-511 is a 1.88 inch (48 mm) wide, 3 mil tape on a 43.7 yard (40 M) roll with a 1.5 inch core and is supplied with a green dispenser / cutter.Catalog number HR4-511 is a 1.88 inch wide, 3 mil tape on a 60 yard roll with a 3 inch core and no dispenser / cutter.Two strips of the HR3-511 or HR4-511 will seal a Corning, Cryschem, CrystalClear Strip, Greiner, Intelliplate, Linbro, Swissci (MRC), VDX and VDXm plate.Catalog number HR4-506 is a 3 inch wide, 3 mil tape on a 54.86 yard roll with a 3 inch core and no dispenser / cutter.One strip of the HR4-506 will seal a Corning, Cryschem M, CrystalClear Strip, Greiner, Intelliplate, Swissci (MRC) and VDXm plate.Crystal Clear Mini Sealing Tape HR4-508 is a 0.75 inch (19 mm) wide, 3 mil tape on a 650 inch (16.5 M) roll and is supplied with a clear dispenser / cutter. The tape is useful for resealing or patching wells after mounting crystals. The tape is also wide enough to seal a single row of a Cryschem S or Cryschem M plate.See chart for tape and plate compatibility.

Hampton CrystalEX Second Generation (Corning)

Hampton CrystalEX Second Generation (Corning)

Crystal<i>EX</i> Second Generation (Corning)

CrystalEx Plates

Applications

   Sitting drop crystallization

Features

96 well sitting drop plate
Round and conical flat bottom drop well shapes
1, 2 or 4 microliter drop wells
1, 3 or 5 drop wells per reservoir
Hydrophilicity treated or untreated
PZero or COC plate material–UV imaging compatible

Description

The second generation of Corning® 96 Well, sitting drop format plates are built to SBS specifications, making them well suited for high throughput crystallization and are fully compatible with robotic equipment. The plates are available in several different configurations with varying drop well shapes, plate materials, and number of drop wells per reagent well. The basic plate design is one reagent well flanked by one or three drop wells, with SBS standard spacing between the centers of adjacent well clusters. One may choose a plate with small (1 µl), medium (2 µl), or large (4 µl) drop well volumes. The choice of round or conical flat well shapes are available. The PZero polymer provides for zero background polarization and is non-birefringent. PZero plates are not treated. The COC polymer offers high chemical resistance. Both types of plastic feature improved transparency. The reservoir numbers are embossed on each individual well for easy identification. Drop well locations conform to SBS standards for robotic handling. The low-volume reagent well saves on reagent costs. The plates can be sealed using Crystal Clear Sealing Film (HR3-609), 3 inch wide Crystal Clear Sealing Tape (HR4-506) or ClearSeal Film™ (HR4-521).

Corning has discontinued 3557 – special pricing and limited inventory available for this item only.

CAT NO NAME DESCRIPTION
HR8-135 Corning 3556 4µl round drop well, 1 drop well, COC, untreated – 10 plate case
HR8-134 Corning 3556 4 µl  round drop well, 1 drop well, COC, untreated – 40 plate case
HR8-137 Corning 3551 4 µl conical flat drop well, 1 drop well, COC, treated – 10 plate case
HR8-136 Corning 3551 4µl conical flat drop well, 1 drop well, COC, treated – 40 plate case
HR8-139 Corning 3552 2 µl round drop well, 3 drop well, COC, untreated – 10 plate case
HR8-138 Corning 3552 2µl  round drop well, 3 drop well, COC, untreated – 40 plate case
HR8-141 Corning 3553 2 µl conical flat drop well, 3 drop well, COC, untreated – 10 plate case
HR8-140 Corning 3553 2 µl conical flat drop well, 3 drop well, COC, untreated – 40 plate case
HR8-147 Corning 3550 1µll conical flat drop well, 3 drop well, COC, untreated – 10 plate case
HR8-146 Corning 3550 1µl conical flat drop well, 3 drop well, COC, untreated – 40 plate case
HR8-160 Corning 3557 1 µl conical flat drop well, 5 drop well, PZero – 10 plate case
HR8-158 Corning 3557 1 µl conical flat dro p well, 5 drop well, PZero – 40 plate case

Hampton Solubility & Stability Screen

Hampton Solubility & Stability Screen

Solubility & Stability Screen(溶解度和稳定性的屏幕)

应用:溶解性和稳定性屏幕的设计,以协助识别解决方案的条件,促进蛋白质的溶解性和稳定性,并尽量减少蛋白质沉淀。

Solubility & Stability Screen

Solubility & Stability Screen

Solubility & Stability Screen

Solubility & Stability Screen HR2-072

Applications

A solubility screen, a stability screen, an additive screen for protein assays including ThermoFluor and crystallization
Preformulation screening

 

Compatible with Thermofluor , Differential Scanning Fluorimetry (DSF), and Protein Thermal Shift assays
96 sterile filtered reagents
17 classes of reagents and excipients
Highly concentrated (10x) reagent formulation
Developed at Hampton Research

Features

Methodologies

Thermal shift assay
Dynamic Light Scattering
Use Solubility & Stability Screen along side Slice pH and Solubility & Stability Screen 2 to optimize sample buffer
Description

Solubility & Stability Screen is designed to assist in the identification of solution conditions which promote protein solubility and stability, and minimize protein precipitation. Solubility & Stability Screen is a solubility screen, a stability screen, and may also be used as an additive screen in the presence of a crystallization reagent.

The Hampton Research Solubility and Stability Screen can evaluate protein solubility, stability and crystallization in the presence of 94 different chemical additives sampling 17 different classes of reagents plus two controls.

Protein solubility and stability are universally required in a wide range of applications, including general biochemical studies, the preparation of proteins in pharmaceuticals, structural biology and crystallization.1-11The preparation of a concentrated, soluble and stable protein sample can often be a difficult task as proteins often aggregate, precipitate or denature.

Thermofluor (also known as Differential Scanning Fluorimetry, DSF and Protein Thermal Shift) provides a rapid, convenient and effective means of identifying subsaturating solution conditions that may also correlate with protein heterogeneity and poor crystallization outcomes.12

Protein solubility and stability is affected by many different chemical factors including pH, buffer type, chemical additives and excipients. pH and buffer type are dominant protein solubility and stability variables and can be evaluated and optimized using the Hampton Research Slice pH kit HR2-070. Slice pH evaluates protein solubility, stability and crystallization versus 20 different buffers over the pH range 3.5-9.6. Chemical additives influencing protein solubility and stability can be evaluated using the Solubility & Stability Screen.

It is widely accepted that protein solubility and stability can be increased by the use of chemical additives.5,15Seventeen classes of reagents are sampled by the Solubility & Stability Screen and each of these classes has been reported as important in improving sample solubility and stability.2-11

The Solubility & Stability Screen is a set of 94 high purity reagents formulated in high purity water (NCCLS/ASTM Type 1+) at 25°C and are 0.22 micron sterile filtered. The 94 solubility and stability reagents are formulated at 2 to 10 times their recommended working concentration. The remaining two reagents are water and a negative (TCA) control. A water control demonstrates the effect of diluting sample as well as sample buffer concentration. TCA, the negative control, demonstrates total sample precipitation, loss of sample solubility and loss of sample stability. The effects of the Solubility & Stability reagents can be compared with this negative control to assist in the identification of reagents promoting sample solubility and stability. 500 microliters of each reagent is supplied in a sterile 96 well polypropylene Deep Well block. The Solubility & Stability Screen reagents are compatible with the sitting or hanging drop vapor diffusion, microbatch, free interface diffusion, sandwich drop vapor diffusion, and dialysis crystallization methods utilizing water soluble reagents.

Hampton 24孔悬滴板 VDX Plate with sealant(带密封剂)

Hampton 24孔悬滴板 VDX Plate with sealant(带密封剂)
VDX Plate with sealant
Applications

Hanging drop crystallization
Sitting drop crystallization with Micro-Bridges® or Glass Sitting Drop Rods™
Dialysis crystallization with Dialysis Buttons™
Features

24 well plate with raised cover
Sealant applied to 24 wells, ready to seal with cover slides
Compatible with 22 mm diameter square and circle cover slides
Optically clear plastic
Extremely versatile and cost-effective crystallization platform
Compatible with UV crystal imaging when used with HR3-231 and HR3-233 – 22 mm x 0.22 mm Siliconized circle cover slides and HR3-215 and HR3-217 – 22 mm x 0.22 mm Siliconized square cover slides

Description
24 well crystallization plate for hanging drop or sitting drop vapor diffusion crystallization (when used with Micro-Bridges or Glass Rods), or dialysis crystallization (when used with Dialysis Buttons). Stackable, optically clear plastic 24 well plates with raised covers (to allow room for cover slides) and flat bottoms for exceptional optics. Raised, wide rings around each reservoir (well) minimize cross contamination and allow each well to be individually sealed with 22 mm diameter circle or square cover slides. Plates are individually wrapped and supplied with applied sealant. 15.0 cm x 10.8 cm plate footprint is convenient for manual pipetting and well access while at the same time compatible with some automated liquid handling systems. Approximate dimensions: 15.0 cm x 10.6 cm x 2.2 cm. Approximate well size: 1.7 cm x 1.6 cm. Typical well volume: 500 to 1,000 µl. Well capacity: 3.5 ml.

CAT NO NAME DESCRIPTION
HR3-172 VDX邃「 Plate with sealant 24 plate case
HR3-170 VDX邃「 Plate with sealant 100 plate case