标签归档：Hampton 蛋白质结晶试剂盒

Hampton Polyethylene glycol 4,000

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Hampton Polyethylene glycol 4,000

Applications
Crystallization grade Polyethylene glycol for formulating screens or for optimizatio
Features
Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description
Polyethylene glycol 4,000
Synonym: PEG 4,000
H(OCH2CH2)nOH
Mr 3,500-4,500
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3500000
MDL Number MFCD00081839
PubChem Substance ID 24890217
Melting point (Starting Material) 58.0-61.0°C
Aldehyde None detected
Peroxide None detected

HR2-529 Measured pH range: 6.9 – 9.5 at 25°C
HR2-529 Measured Conductivity Range: 35.3 – 46.9 µS/cm at 25°C
HR2-529 Measured Refractive Index Range: 1.40172 – 1.40279 at 20°C

CAT NO NAME DESCRIPTION
HR2-605 Polyethylene glycol 4,000 Flake – 500 g
HR2-529 Polyethylene glycol 4,000 50% w/v solution – 200 ml

Hampton 水晶透明密封带Crystal Clear Sealing Tape

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Hampton 水晶透明密封带Crystal Clear Sealing Tape

Crystal Clear Sealing Tape

3 inch, 1.88 inch, 1.88 inch with dispenser, 0.75 inch with dispenser (left to right)

Crystal Clear Sealing Tape

0.75 inch Crystal Clear Sealing Tape

Applications  应用
Sealing tape used to seal sitting drop crystallization experiments

Description

These optically clear tapes are compatible with protein crystallization reagents. The polypropylene Crystal Clear Sealing Tapes use acrylic solvent based adhesives which are compatible with aqueous crystallization reagents.

Catalog number HR3-511 is a 1.88 inch (48 mm) wide, 3 mil tape on a 43.7 yard (40 M) roll with a 1.5 inch core and is supplied with a green dispenser / cutter.Catalog number HR4-511 is a 1.88 inch wide, 3 mil tape on a 60 yard roll with a 3 inch core and no dispenser / cutter.Two strips of the HR3-511 or HR4-511 will seal a Corning, Cryschem, CrystalClear Strip, Greiner, Intelliplate, Linbro, Swissci (MRC), VDX and VDXm plate.Catalog number HR4-506 is a 3 inch wide, 3 mil tape on a 54.86 yard roll with a 3 inch core and no dispenser / cutter.One strip of the HR4-506 will seal a Corning, Cryschem M, CrystalClear Strip, Greiner, Intelliplate, Swissci (MRC) and VDXm plate.Crystal Clear Mini Sealing Tape HR4-508 is a 0.75 inch (19 mm) wide, 3 mil tape on a 650 inch (16.5 M) roll and is supplied with a clear dispenser / cutter. The tape is useful for resealing or patching wells after mounting crystals. The tape is also wide enough to seal a single row of a Cryschem S or Cryschem M plate.See chart for tape and plate compatibility.

Hampton50号毛细管Glass Number 50 Capillaries

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Hampton50号毛细管Glass Number 50 Capillaries

Glass Number 50 Capillaries

应用：

  X-ray data collection

  Liquid-liquid diffusion crystallization

  Gel acupuncture crystallization

Features

  Glass Number 50 (borosilicate)

  Thin walled – 10 micron

      上海金畔生物作为Hampton蛋白结晶产品的代理商，竭诚为您服务，欢迎新老客户咨询。

Description 描述
Glass Number 50 (borosilicate glass) capillaries that are extremely thin walled (approximately 10 micron wall thickness). The length of the capillary has a well defined diameter, with one end having a funnel shape and the other end closed. Glass capillaries have a wall thickness of 0.01 mm and an overall length of 80 mm +/- 5 mm. Glass capillaries are available in a wide range of outside diameters from 0.1 mm to 2.0 mm. They are designed to mount, hold, and store small molecule and biological macromolecular crystals for x-ray data collection. Capillaries can also be used for crystal density measurements and crystal growth experiments. The capillaries can be sealed tightly against moisture and gases using wax, epoxy, or other sealing materials.

In determining what glass or quartz capillary is right for you, please refer to the “Linear Absorption Coefficient µ cm-1” table. This table indicates the amount of radiation that is absorbed by the capillary during x-ray data collection.

For 0.1 mm to 2.0 mm capillaries the open end capillary tube base size is 3.0 +/- 0.15 mm OD x 0.13 +/- 0.10 mm Wall thickness.

The Diameter is measured 40 mm from the closed end. The tolerances are as follows

Glass Number 50 Capillaries

Glass Number 50 Capillaries

 

0.1 mm -0.075 / +0.075 mm
0.2 mm -0.050 / +0.050 mm
0.3 mm -0.050 / +0.100 mm
0.4 mm -0.100 / +0.100 mm
0.5 mm -0.100 / +0.100 mm
0.6 mm -0.100 / +0.100 mm
0.7 mm -0.150 / +0.150 mm
0.8 mm -0.150 / +0.150 mm
0.9 mm -0.150 / +0.150 mm
1.0 mm -0.150 / +0.250 mm
1.5 mm -0.250 / +0.250 mm
2.0 mm -0.250 / +0.250 mm

Capillaries have only been tested at atmospheric pressure (760 mmHg (torr), 29.92 inHg, 14.696 psi). Use at other pressures has not been tested.

Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理（活性荧光染料）   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理（蛋白结晶） whatman代理（滤膜滤纸） GE代理（蛋白纯化） Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理（糖蛋白分析产品）  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

Hampton 48孔坐滴蛋白结晶板 48-Well Crystallization Plate

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Hampton 48孔坐滴蛋白结晶板 48-Well Crystallization Plate
Applications

Sitting drop crystallization
Features

One drop per reservoir
SBS format
48 well plate
9 mm standard distance between wells
Drop volume: Up to 10 µl
Reservoir volume: 50 to 200 µl
Micro-numbering alongside drop volumes
Rigid plate structure
Wide partition walls between wells improve sealing
Developed in conjunction with the MRC Laboratory of Molecular Biology in Cambridge, United Kingdom
UV compatible (UVP)
Description
MRC Maxi 48-Well Crystallization Plate for Automated Optimization

The MRC Maxi optimization plate is a breakthrough for macromolecular crystallization presented in a 48 well format. Offering easy to automate crystallization optimization with large sitting-drops, the new MRC Maxi Crystallization plate is the perfect solution. Manufactured by Swissci AG, the plate offers an SBS format while providing 48 wells. MRC Maxi is intended for large drops and is compatible both with standard robotic systems as well as manual pipetting.

The plate was developed at the MRC Laboratory of Molecular Biology (Cambridge, UK) in collaboration with Jan Löwe and Fabrice Gorrec. It is a result of many years of experience in successful robotic high-throughput crystallization and complements the original MRC crystallization plate, which is intended for smaller drop volumes and higher throughput during screening.

Drop volumes of up to 10 µl are possible. The 9 mm standard distance between wells is preserved, enabling the use of multi-channel manual pipettes and robotic liquid handlers, making MRC Maxi one of the most automation-friendly optimization plates on the market.

MRC Maxi is covered by global intellectual property and design registration as are the Swissci AG MRC 2 lens 96 well plates. Several breakthrough features of the original MRC plate have been maintained. Wells are labeled individually. Drops are raised for easy access during crystal retrieval. MRC Maxi uses the same proprietary polymer specially selected for the purpose of UV light visualization and the material used keeps through-plastic evaporation to a minimum. Well shapes are spherical but shallow.

The MRC Maxi Crystallization plate offers unique properties that make it the ideal choice for microliter-sized optimization experiments and is made from UV compatible UVP.

The advantages of the MRC Maxi Crystallization plate – in brief

Easy Crystal Retrieval
Raised wide wells make the crystal mounting especially easy.

Easy Viewing
The wells are wide conical.
Each well has a micro lens for perfect illumination.
Micro numbering readable under the microscope for each well.
The optically superior polymer (UVP) is UV transmissible.

Better Sealing
Wide partition walls between the wells give plenty of area for good sealing with tape.

Very Rigid, Automation-Friendly Plate Design
The UVP polymer reduces through-plastic evaporation to a minimum.

SBS Standard
The plates are designed to the SBS standard and are compatible with all common holders.
9 mm distance from well-to-well within columns, 18 mm distance within rows.

Unique Polymer (UVP)
Ultra-low sample binding.
No static charging.

Recommended Volumes
Volumes validated for MRC Maxi are up to 10 µl of sample drop and 200 µl of the crystallization reagent.
CAT NO NAME DESCRIPTION
HR3-179 MRC Maxi 48-Well Crystallization Plate 10 plate case
HR3-180 MRC Maxi 48-Well Crystallization Plate 40 plate case

Hampton CrystalEX Second Generation (Corning)

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Hampton CrystalEX Second Generation (Corning)

Crystal<i>EX</i> Second Generation (Corning)

CrystalEx Plates

Applications

   Sitting drop crystallization

Features

96 well sitting drop plate
Round and conical flat bottom drop well shapes
1, 2 or 4 microliter drop wells
1, 3 or 5 drop wells per reservoir
Hydrophilicity treated or untreated
PZero or COC plate material–UV imaging compatible

Description

The second generation of Corning® 96 Well, sitting drop format plates are built to SBS specifications, making them well suited for high throughput crystallization and are fully compatible with robotic equipment. The plates are available in several different configurations with varying drop well shapes, plate materials, and number of drop wells per reagent well. The basic plate design is one reagent well flanked by one or three drop wells, with SBS standard spacing between the centers of adjacent well clusters. One may choose a plate with small (1 µl), medium (2 µl), or large (4 µl) drop well volumes. The choice of round or conical flat well shapes are available. The PZero polymer provides for zero background polarization and is non-birefringent. PZero plates are not treated. The COC polymer offers high chemical resistance. Both types of plastic feature improved transparency. The reservoir numbers are embossed on each individual well for easy identification. Drop well locations conform to SBS standards for robotic handling. The low-volume reagent well saves on reagent costs. The plates can be sealed using Crystal Clear Sealing Film (HR3-609), 3 inch wide Crystal Clear Sealing Tape (HR4-506) or ClearSeal Film™ (HR4-521).

Corning has discontinued 3557 – special pricing and limited inventory available for this item only.

CAT NO NAME DESCRIPTION
HR8-135 Corning 3556 4µl round drop well, 1 drop well, COC, untreated – 10 plate case
HR8-134 Corning 3556 4 µl  round drop well, 1 drop well, COC, untreated – 40 plate case
HR8-137 Corning 3551 4 µl conical flat drop well, 1 drop well, COC, treated – 10 plate case
HR8-136 Corning 3551 4µl conical flat drop well, 1 drop well, COC, treated – 40 plate case
HR8-139 Corning 3552 2 µl round drop well, 3 drop well, COC, untreated – 10 plate case
HR8-138 Corning 3552 2µl  round drop well, 3 drop well, COC, untreated – 40 plate case
HR8-141 Corning 3553 2 µl conical flat drop well, 3 drop well, COC, untreated – 10 plate case
HR8-140 Corning 3553 2 µl conical flat drop well, 3 drop well, COC, untreated – 40 plate case
HR8-147 Corning 3550 1µll conical flat drop well, 3 drop well, COC, untreated – 10 plate case
HR8-146 Corning 3550 1µl conical flat drop well, 3 drop well, COC, untreated – 40 plate case
HR8-160 Corning 3557 1 µl conical flat drop well, 5 drop well, PZero – 10 plate case
HR8-158 Corning 3557 1 µl conical flat dro p well, 5 drop well, PZero – 40 plate case

Hampton GRAS 1 • GRAS 2 Screens

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Hampton GRAS 1 • GRAS 2 Screens

stallization screen for proteins, including monoclonal antibodies, where Polyethylene glycol is the primary, and Salt the secondary reagent, sampling pH 4-9
GRAS 1 • GRAS 2 ScreensGRAS 1 • GRAS 2 Screens Identify GRAS based reagents that promote crystallization of biotherapeutics for bioprocess, bioformulation, and continuous flow manufacturing applications
Features

Developed at Hampton Research
Generally Recognized As Safe reagent formulation
Samples pH 4.0 – 9.0 without an added buffer
Primary crystallization reagent:

GRAS Screen 1: PEG 300, 400, MME 550, & 600
GRAS Screen 2: PEG 1,000, MME 2,000, 3,350 & 4,000
Secondary crystallization reagent:

24 unique salts
Compatible with Vapor diffusion, microbatch, free interface diffusion
Reagents soluble between 4°C and 30°C

Description
GRAS Screen™ 1 and GRAS Screen™ 2 were developed by Hampton Research for the crystallization of proteins, including monoclonal antibodies. Each of the chemicals in GRAS Screen 1 and 2 have been used under one or more of the following categories. As (1) a Generally Recognized As Safe (GRAS) substance, (2) a pharmaceutical excipient, (3) a normal physiological constituent, (4) a metabolic byproduct, and/or (5) a Everything Added to Food in the United States (EAFUS) substance.

GRAS Screen 1 samples four low molecular weight Polyethylene glycols (300, 400, MME 550, and 600) versus twenty-four salts, encompassing pH 4-9. GRAS Screen 1 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 1 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

GRAS Screen 2 samples four medium molecular weight Polyethylene glycols (1,000, MME 2,000, 3,350, & 4,000) versus twenty-four salts, encompassing pH 4-9. GRAS Screen 2 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 2 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.
ITEMS
CAT NO NAME DESCRIPTION
HR2-451 GRAS Screen 1 1 ml, Deep Well block format
Price Quantity
$245.00
CAT NO NAME DESCRIPTION
HR2-452 GRAS Screen 2 1 ml, Deep Well block format
Price Quantity
$245.00

Hampton 96孔坐滴蛋白结晶板–Intelli-Plate

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Hampton 96孔坐滴蛋白结晶板–Intelli-Plate

在生命科学领域，上海金畔生物为业界提供了丰富的实验室和生物医药生产研发的产品。庆祝上海金畔生物正式成为Hampton Intelli-Plate 96孔蛋白结晶板的签约代理商，在这里要感谢广大客户多年来对上海金畔生物科技有限公司的支持和厚爱，我们将一如既往的为广大客户带来Hampton Intelli-Plate 96孔蛋白结晶板高品质的产品和服务，欢迎广大新老客户来电咨询。

Intelli-Plate 96 (Art Robbins Instruments)

Applications

  Sitting Drop Crystallization   用于蛋白坐滴结晶

Features  特征：

SBS microplate footprint & standard well spacing
Optically clear wells provide for superior crystal imaging
Well layout makes crystal harvesting easier
UV transmissible and low birefringence
2 well versions available in small and large drop well or two identical shallow drop wells
3 well versions are ideal for optimizing protein concentration, additive screens, drop ratio and combinatorial experiments
Rounded reservoir corners to prevent reservoir (MPD) creep

Description

Art Robbins Instruments has developed the ideal family of plates for crystallography applications: the Intelli-Plate. All versions of the Intelli-Plate have the SBS microplate footprint and standard well spacing and are ideal for manual or automated processing.

The Intelli-Plate 96-2 Low Profile (Art Robbins 102-0001-10 Hampton Research HR3-116 and HR3-117) is a low profile version of the Intelli-Plate 96-2 LVR (Low Volume Reservoir). Reservoir volume: 100 µl max. Drop well volume: 4 µl and 10 µl max. UV transmissible and low birefringence.

The CrystalMation Intelli-Plate 96-3 low-profile crystallization plate (Art Robbins 102-0001-13 Hampton Research HR3-118 and HR3-119) is a low profile version of the Intelli-Plate 96-3 LVR (Low Volume Reservoir). The plate is designed for sitting drop vapor diffusion crystallization experiments. It is constructed from optically clear, UV-transmissible, chemically resistant plastic with superior low birefringence. The low-profile construction allows for higher density in plate storage and imaging systems, maximizing your investment and valuable space. The plate is designed to be a substantial improvement in existing low-profile plates, allowing for superior sealing due to thicker well design and a flat sealing surface. It has been built to the SBS (Society for Biomolecular Screening) standard dimensions with 8 vertical wells along the left side of the plate (A-H) and 12 horizontal wells along the top of the plate (1-12). It is compatible with automated instrumentation including the CrystalMation line of instruments from Rigaku. The plate features three identical sized sample drop wells per reservoir. The wells are concave depressions along the left side (Y-axis) of the plate and are located on the ledge above the adjacent, flat bottom reagent reservoir. Each well features a round bottom for easy crystal harvesting and can hold up to 1 µl of sample. The multiple well design allows for combinatorial experiments, complex co-crystallization experiments for ligand screening, or for drop concentration variation. The reagent reservoir is typically filled with 50 to 70 µl of reagent. Reservoir volume: 100 µl max. Drop well volume: 1 µl max. UV transmissible and low birefringence.

The Intelli-Plate 96-2 LVR (Art Robbins 102-0001-00 Hampton Research HR3-143 and HR3-145) is a Low Volume Reservoir version of the original Intelli-Plate. Reservoir volume: 100 µl max. Drop well volume: 1 µl max. UV transmissible and low birefringence.

The Intelli-Plate 96-3 LVR (Art Robbins 102-0001-03 Hampton Research HR3-183 and HR3-185) is a Low Volume Reservoir, 3 drop well version of the Intelli-Plate. Reservoir volume: 100 µl max. Typical reservoir volume 50 µl. Drop well volume: 1 µl max. UV transmissible and low birefringence.

The Intelli-Plate 96-2 Original crystallization plate (Art Robbins 102-0011-00 Hampton Research HR3-297 and HR3-299) is an optically clear plate for sitting drop vapor diffusion crystallization. All wells have standard 9 mm spacing to conform to SBS standards with 8 vertical wells along the left side of the plate (A-H) and 12 horizontal wells along the top of the plate (1-12). This plate features two locations for sample per reservoir. The sample drop locations are located along the left side of the reagent reservoir, along the Y-axis of the plate. The sample wells are concave depressions on the ledge above the adjacent, flat bottom reagent reservoir. One sample well is located above the second sample well. The top sample well can hold 10 µl or less of sample. The lower sample well can hold 4 µl or less of sample. The reagent reservoir is typically filled with 100 µl of reagent and is capable of holding up to 300 µl of reagent. The sidewalls separating adjacent wells or reservoirs are 0.9 and 1.0 mm thick in order to offer a larger area for sealing the plate and a separation of the reservoirs. This plate is rigid, with virtually no torsional flex and is designed for either manual or automated pipetting. UV transmissible and low birefringence.

The Intelli-Plate Flat Shelf (Art Robbins 102-0001-01 Hampton Research HR8-171 and HR8-172) crystallization plate is a flat drop shelf version of the original Intelli-Plate. Reservoir volume: 300 µl max. No drop well. UV transmissible and low birefringence.

The Intelli-Plate 96-2 Shallow Well (Art Robbins 102-0001-20 Hampton Research HR3-163 and HR3-164) has two identically sized 2 µL reaction wells and a 140 µL screen reservoir. Each of the two identical well features a round bottom shallow well design for easy crystal harvesting and is arranged in an 8 x 12 array. This plate has low birefringence and is UV compatible. This plate is also available in a low profile height (Art Robbins 102-0001-21).

The plates can be sealed using Crystal Clear Sealing Film (HR3-609), 3 inch wide Crystal Clear Sealing Tape (HR4-506) or ClearSeal Film™ (HR4-521).

The height of Intelli-Plate is 0.560 inches. The height of Intelli-Plate Low Profile is 0.315 inches (8.001 mm).

Intelli-Plate 96 (Art Robbins Instruments)

Intelli-Plate 96-3

CAT NO NAME DESCRIPTION
HR3-116 Intelli-Plate 96-2 Low Profile 20 plate case
HR3-117 Intelli-Plate 96-2 Low Profile 80 plate case
HR3-118 CrystalMation Intelli-Plate 96-3 low-profile 20 plate case
HR3-119 CrystalMation Intelli-Plate 96-3 low-profile 80 plate case
HR3-143 Intelli-Plate 96-2 LVR 10 plate case
HR3-145 Intelli-Plate 96-2 LVR 40 plate case
HR3-183 Intelli-Plate 96-3 LVR 10 plate case
HR3-185 Intelli-Plate 96-3 LVR 40 plate case
HR3-297 Intelli-Plate 96-2 Original 10 plate case
HR3-299 Intelli-Plate 96-2 Original 40 plate case
HR8-171 Intelli-Plate Flat Shelf 10 plate case
HR8-172 Intelli-Plate Flat Shelf 40 plate case
HR3-163 Intelli-Plate 96-2 Shallow Well 10 plate case
HR3-164 Intelli-Plate 96-2 Shallow Well 40 plate case
HR3-181 Intelli-Plate 96-2 Shallow Well Low Profile 20 plate case
HR3-182 Intelli-Plate 96-2 Shallow Well Low Profile 80 plate case

Sigma代理  Abcam代理  CST代理  Santa Cruz代理  Biolegend代理 ebioscience代理  Invitrogen代理   millipore代理  BD流式抗体代理   GeneTe x抗体代理  Novus抗体代理   R&D代理 Biovison代理  Jackson代理  MBL抗体代理  ProSpec抗体代理  Bethyl抗体代理  Antibody Revolution抗体代理  Torrey Pines Biolabs代理  Amresco代理  MPbio代理  Laysan bio代理  NANOCS代理  Avanti代理  wako代理  lumiprobe代理（活性荧光染料）   NEB酶代理  Roche酶代理  Toyobo酶代理   USP代理  EP代理  Dr代理  TRC代理  TCI代理  Reagecon代理 Megazyme代理 Hampton代理（蛋白结晶） whatman代理（滤膜滤纸） GE代理（蛋白纯化） Corning康宁代理  Axygen代理 Falcon代理  NISSUI日水代理 Himedia代理 OXOID代理  BD培养基代理 Ludger代理（糖蛋白分析产品）  Eppendorf代理  Labnet代理  标准品代理 抗体代理 酶试剂代理  培养基代理 耗材代理 Elisa试剂盒代理 。

Hampton 银弹 Silver Bullets

发表于由

Hampton 银弹 Silver Bullets

Applications

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Developed at Hampton Research
Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

Description
Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3

Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3

X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.

Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests

The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:

•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal

The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ºC, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.

Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.

Silver Bullets reagent volume is 0.5 ml (each well).

ThermoFluor is a registered trademark of Johnson & Johnson.

Hampton  Silver Bullets Bio

Silver Bullets • Silver Bullets Bio

Silver Bullets • Silver Bullets Bio

上海金畔生物作为Hampton蛋白结晶产品的代理商，竭诚为您服务，欢迎新老客户咨询。

Applications 应用

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

 
Developed at Hampton Research
Two sets of 96 reagents, composed of more than 1,090 chemicals, of which more than 400 are uniqu

Please note, the Silver Bullets Bio Screen is temporarily unavailable as a new formulation is in development

 

Hampton Natrix • Natrix 2 • Natrix HT

发表于由

Hampton Natrix • Natrix 2 • Natrix HT

Applications

  Primary biased sparse matrix crystallization screen for nucleic acids & protein/nucleic acid complexes  主要用于核酸和蛋白质/核酸复合物的删选

Features

Nucleic acid sparse matrix screen
Sparse matrix formulation efficiently samples salts, polyols, polymers, organics, additives & pH
pH range 5.6 – 8.5
Tube or Deep Well block format
 Description
Natrix, Natrix 2 and Natrix HT are based upon published reagent formulations for the crystallization of nucleic acids and protein-nucleic acid complexes. A variety of hammerhead ribozymes and other ribozymes, RNAs, DNAs, RNA-drug complexes, and RNA-protein complexes have been crystallized using the Natrix protocols.By using sparse matrix sampling technology, The Natrix kits allow one to quickly test wide ranges of pH, salts, and precipitants using a very small sample of nucleic acid.

Natrix screens are unique in that rather than relying solely on the traditional nucleic acid precipitant MPD, Natrix screens also utilize Polyethylene glycols (PEGs) in a variety of molecular weights (200, 400, 4,000, 8,000) as well as 2-Propanol, Polyethylene glycol monomethyl ether (PEG MME), and 1,6-Hexanediol. Many of the polymeric and low molecular weight organic precipitants are combined with various monovalent salts as precipitating agents. This combination of salts and low molecular weight organics and polyalcohols, as well as the utilization of varying chain length PEGs, has proven to be a successful combination for producing nucleic acid and protein-nucleic acid complex crystals.

Natrix contains 48 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by William Scott in 1995.

Natrix 2, an extension of Natrix, contains 48 unique reagents, 10 ml each. Natrix 2 is a biased sparse matrix screen based on extracting patterns from crystallization data as well as reagent formulations first described by Berger et al in 1996.

Natrix HT contains 1 ml of each reagent from Natrix and Natrix 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
Crystals of a frameshift promoting RNA pseudoknot from the coronavirus IBV, grown using the Hampton Research Natrix screen. Simon Pennell, MRC National Institute for Medical Research, United Kingdom.
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Natrix • Natrix 2 • Natrix HT
Crystals of Ec Rnk, a new RNA polymerase interacting protein obtained in Hampton Research Natrix Screen. Valerie Lamour, IGBMC, Illkirch, France.

 

CAT NO NAME DESCRIPTION
HR2-116 Natrix 10 ml, tube format
HR2-117 Natrix 2 10 ml, tube format
HR2-131 Natrix HT 1 ml, Deep Well block format